Adenylate Cyclase (ADCY) (Active) Protein

Catalog No. ABIN2452164

Product Details

Target: Adenylate Cyclase (ADCY)

Protein Type: Recombinant

Biological Activity: Active

Origin: Bordetella pertussis

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func), SDS-PAGE (SDS), ELISA, Western Blotting (WB)

Purity: > 90 % purity by SDS-PAGE (CBB staining)

Application Details

Application Notes: Functionale studies on the cAMP- signaling pathway (10~100 ng/mL)
2) Antigen for western blotting and ELISA
3) SDS-PAGE marker as native adenylate cyclase Activity tested: Addition of the 10~100 ng/mL adenylate cyclase toxin to medium increased significant levels of cellular cAMP levels in CHO and human monocyte cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 550 µg/mL

Buffer: 50 mM Tris-HCl ( pH 8.0), 6 M urea, 150 mM NaCl

Storage: -80 °C

References

Westrop, Hormozi, Da Costa, Parton, Coote: "Bordetella pertussis adenylate cyclase toxin: proCyaA and CyaC proteins synthesised separately in Escherichia coli produce active toxin in vitro." in: Gene, Vol. 180, Issue 1-2, pp. 91-9, (1997) (PubMed).

Friedman: "Bordetella pertussis adenylate cyclase: isolation and purification by calmodulin-sepharose 4B chromatography." in: Infection and immunity, Vol. 55, Issue 1, pp. 129-34, (1987) (PubMed).

Target Details

Target: Adenylate Cyclase (ADCY)

Background: Adenylate cyclase is a toxin produced by a gram-negative coccobacillus, Bordetella pertusis. This toxin penetrates into the animal cells and produces cAMP from ATP in the cytoplasm. It belongs to the RTX family of toxins produced by many gram-negative bacteria. The molecular weight is 177 kDa This product is a recombinant toxin of Bordetella pertussis Tohama strain expressed in E. coli from the structural gene cyaA and its activator gene cyaC and purified by the method of Friedman.

UniProt: P0DKX7