VEGF121 Protein (AA 27-147)

Catalog No. ABIN2181899

Product Details

Target: VEGF121 (Vascular Endothelial Cell Growth Factor 121 (VEGF121))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 27-147

Origin: Human

Source: HEK-293 Cells

Brand: ActiveMax®

Sequence: AA 27-147

Characteristics: This protein carries no "tag". The protein has a calculated MW of 14 kDa (monomer). As a result of glycosylation, the protein migrates as 19 kDa and 20 kDa under reducing (R) condition, and 40-45 kDa (homodimer) under non-reducing (NR) condition (SDS-PAGE).

Purity: >95 % as determined by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).

References

Stafford, Matsumoto, Yin, Cai, Fung, Stephenson, Gill, You, Lin, Wang, Masikat, Li, Penta, Steiner, Baliga, Murray, Thanos, Hallam, Sato: "In vitro Fab display: a cell-free system for IgG discovery." in: Protein engineering, design & selection : PEDS, Vol. 27, Issue 4, pp. 97-109, (2014) (PubMed).

Target Details

Target: VEGF121 (Vascular Endothelial Cell Growth Factor 121 (VEGF121))

Alternative Name: VEGF-121 (VEGF121 Products)

Background: Vascular endothelial growth factor (VEGF) is also known as vascular permeability factor (VPF) and VEGF-A, and is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. Alternatively spliced isoforms of 121,145,165,183,189 and 206 amino acids in length are expressed in humans. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189. VEGF121 is the only form that lacks a basic heparinbinding region and is freely diffusible. Mouse embryos expressing only the corresponding isoform (VEGF120) do not survive to term, and show defects in skeletogenesis. Human VEGF121 shares 87 % aa sequence identity with corresponding regions of mouse and rat, 93 % with feline, equine and bovine, and 91 %, 95 % and 96 % with ovine, canine and porcine VEGF, respectively. VEGF121 induces the proliferation of lymphatic endothelial cells. The lymphangiogenesis may be promoted by upregulation of VEGF121, which may in turn act in part via induction of VEGF-C.

Molecular Weight: 14.1 kDa

NCBI Accession: NP_001165099