TNFRSF4 Protein (AA 29-216) (His tag)

Catalog No. ABIN2181571

Product Details

Target: TNFRSF4 (Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 29-216

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This TNFRSF4 protein is labelled with His tag.

Sequence: AA 29-216

Characteristics: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 22 kDa. The protein migrates as 40-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Purity: >90 % as determined by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).

References

Upadhyaya, Lahdenranta, Hurov, Battula, Dods, Haines, Kleyman, Kristensson, Kublin, Lani, Ma, Mudd, Repash, Van Rietschoten, Stephen, You, Harrison, Chen, McDonnell, Brandish, Keen: "Anticancer immunity induced by a synthetic tumor-targeted CD137 agonist." in: Journal for immunotherapy of cancer, Vol. 9, Issue 1, (2021) (PubMed).

Simons, Lim, Carter, Wagner, Wayham, Adler, Johnson: "Affinity maturation of antibodies by combinatorial codon mutagenesis versus error-prone PCR." in: mAbs, Vol. 12, Issue 1, pp. 1803646, (2020) (PubMed).

Fromm, de Silva, Johannes, Patel, Hornblower, Schreiber: "Agonist redirected checkpoint, PD1-Fc-OX40L, for cancer immunotherapy." in: Journal for immunotherapy of cancer, Vol. 6, Issue 1, pp. 149, (2018) (PubMed).

Target Details

Target: TNFRSF4 (Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4))

Alternative Name: OX40 (TNFRSF4 Products)

Synonyms: TNFRSF4 Protein, ACT35 Protein, CD134 Protein, Ly-70 Protein, Ox40 Protein, TXGP1L Protein, Txgp1 Protein, OX40 Protein, TNF receptor superfamily member 4 Protein, tumor necrosis factor receptor superfamily, member 4 Protein, TNFRSF4 Protein, Tnfrsf4 Protein

Background: Tumor necrosis factor receptor superfamily member 4 (TNFRSF4) is also known as ACT35 antigen, OX40L receptor, TAX transcriptionally-activated glycoprotein 1 receptor, CD antigen CD134, OX40. OX40 / TNFRSF4 contains four TNFR-Cys repeats. TNFRSF4 is receptor for TNFSF4 / OX40L / GP34 and can interacts with TRAF2, TRAF3 and TRAF5.

Molecular Weight: 22.0 kDa

NCBI Accession: NP_003318

Pathways: Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints