CCL17 Protein

Catalog No. ABIN2018391

Product Details

Target: CCL17 (Chemokine (C-C Motif) Ligand 17 (CCL17))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Sequence: ARATNVGREC CLDYFKGAIP IRKLVSWYKT SVECSRDAIV FLTVQGKLIC ADPKDKHVKK AIRLVKNPRP

Characteristics: Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 1.0-10 ng/mL.

Purity: > 97 % by SDS-PAGE and HPLC analyses.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1 EU/μg of rMuTARC/CCL17 as determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Buffer: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C.

Target Details

Target: CCL17 (Chemokine (C-C Motif) Ligand 17 (CCL17))

Alternative Name: TARC/CCL17 (CCL17 Products)

Synonyms: A-152E5.3 Protein, ABCD-2 Protein, SCYA17 Protein, TARC Protein, CCL17 Protein, Abcd-2 Protein, Scya17 Protein, Scya17l Protein, Tarc Protein, abcd2 Protein, C-C motif chemokine ligand 17 Protein, chemokine (C-C motif) ligand 17 Protein, ATP-binding cassette sub-family D member 2 Protein, CCL17 Protein, Ccl17 Protein, LOC100537809 Protein

Background: Thymus and activation regulated chemokine (TARC) is a novel CC chemokine, also called CCL17, recently identified using a signal sequence trap method. CCL17 cDNA encodes a highly basic 94 amino acid (a.a.) residue precursor protein with a 23 a.a. residue signal peptide that is cleaved to generate the 71 a.a. residue mature secreted protein. Among CC chemokine family members, CCL17 has approximately 24 - 29 % amino acid sequence identity with RANTES, MIP-1alpha, MIP-1beta, MCP-1, MCP-2, MCP-3 and I-309. CCL17 is constitutively expressed in thymus, and at a lower level in lung, colon, and small intestine. CCL17 is also transiently expressed in stimulated peripheral blood mononuclear cells. Recombinant CCL17 has been shown to be chemotactic for T cell lines but not monocytes or neutrophils. CCL17 was recently identified to be a specific functional ligand for CCR-4, a receptor that is selectively expressed on T cells.
Synonyms: C-C motif chemokine 17, Small-inducible cytokine A17, Thymus and activation-regulated chemokine, CC chemokine TARC, ABCD-2, SCYA17, A-152E5.3, MGC138271, MGC138273.

Molecular Weight: 7.9 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids.