Sonic Hedgehog Protein (SHH) (AA 25-198, Cys25IleIle-Mutant)
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- Target See all Sonic Hedgehog (SHH) Proteins
- Sonic Hedgehog (SHH)
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 25-198, Cys25IleIle-Mutant
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Origin
- Mouse
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Source
- Escherichia coli (E. coli)
- Characteristics
- ED50<1.0 μg/mL, measured by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) Cells, corresponding to a specific activity of>1.0× 10^3 units/mg.
- Purity
- > 95 % by SDS-PAGE and HPLC analyses.
- Endotoxin Level
- < 0.2 EU/μg, determined by LAL method.
- Top Product
- Discover our top product SHH Protein
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Sonic Hedgehog (SHH) (AA 24-197), (Cys24IleIle-Mutant) (Active) protein
SHH Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active
Sonic Hedgehog (SHH) (AA 24-197) proteinSHH Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sonic Hedgehog (SHH) protein (His tag)SHH Origin: Human Host: HEK-293 Cells Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
Sonic Hedgehog (SHH) protein (His tag)SHH Origin: Mouse Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE.
Sonic Hedgehog (SHH) (AA 25-198) protein (His tag)SHH Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS-PAGE SDS
Sonic Hedgehog (SHH) (AA 24-197) protein (His tag)SHH Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS - PAGE SDS
Sonic Hedgehog (SHH) (Active) proteinSHH Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE Active
Sonic Hedgehog (SHH) (AA 181-280) protein (GST tag)SHH Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Sonic Hedgehog (SHH) (Active) protein (His tag)SHH Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
Sonic Hedgehog (SHH) (AA 24-197) protein (His tag)SHH Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstituted in ddH2O at 100 μg/mL.
- Buffer
- Lyophilized after extensive dialysis against PBS.
- Storage
- -80 °C
- Storage Comment
- Lyophilized recombinant mouseSonic Hedgehog(rmShh)remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmShhshould be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
- Expiry Date
- 6 months
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- Target
- Sonic Hedgehog (SHH)
- Abstract
- SHH Products
- Background
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Sonic Hedgehog (Shh) is a member of the Hedgehog (Hh) family of highly conserved proteins which are widely represented throughout the animal kingdom. In mammal, there are threerelatedHh proteins, Sonic (Shh), Desert (Dhh) and Indian (Ihh). They share a high degree of amino-acid sequence identity (e.g., Shh and Ihh are 93 % identical). Sonic Hedgehog plays a role in cell growth, cell specialization, and the normal shaping (patterning) of the body. Shh is also important for development of the brain and spinal cord (central nervous system), eyes, limbs, and many other parts of the body. Recombinant mouseSonic Hedgehog(rmShh) produced in E.coli is a single non-glycosylated polypeptide chain containing 176 amino acids. A fully biologically active molecule, rmShh,is obtained by proprietary chromatographic techniques with a molecular mass of 19.8 kDa analyzed by reducing SDS-PAGE.
Synonyms: Hhg1 - Molecular Weight
- 19.8kDa, observed by reducing SDS-PAGE.
- UniProt
- Q62226
- Pathways
- Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
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