IL6RA Protein (AA 20-358, Isoform 1) (His tag)
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- Target See all IL6RA Proteins
- IL6RA (Interleukin 6 Receptor, alpha (IL6RA))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- Isoform 1, AA 20-358
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Origin
- Human
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Source
- Insect cells (Sf9)
- Purification tag / Conjugate
- This IL6RA protein is labelled with His tag.
- Characteristics
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ED50 < 50 ng/mL, measured by the cytotoxicity assay using M1 cells in presence of 10 ng/mL of human IL-6, corresponding to a specific activity of > 2x10^4 units/mg.
AA 20-358 (isoform 1), expressed with an N-terminal Histag. - Purity
- > 95 % by SDS-PAGE and HPLC analyses.
- Endotoxin Level
- < 0.2 EU/μg, determined by LAL method.
- Top Product
- Discover our top product IL6RA Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstituted in ddH2O at 100 μg/mL.
- Buffer
- Lyophilized after extensive dialysis against PBS.
- Storage
- -80 °C
- Storage Comment
- Lyophilized recombinant human Soluble IL-6 Receptor Alpha (rhsIL-6R alpha) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhsIL-6R alpha remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.
- Expiry Date
- 6 months
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- Target
- IL6RA (Interleukin 6 Receptor, alpha (IL6RA))
- Abstract
- IL6RA Products
- Synonyms
- IL6R1 Protein, Il6ra Protein, CD126 Protein, IL-6R-1 Protein, IL-6RA Protein, IL6Q Protein, IL6RA Protein, IL6RQ Protein, gp80 Protein, IL-6R Protein, Il6r Protein, interleukin 6 receptor Protein, interleukin 6 receptor, alpha Protein, Il6r Protein, IL6R Protein, Il6ra Protein
- Background
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Interleukin-6 Receptor (IL-6R) is a single trans-membrane protein that is the receptor for Interleukin-6 (IL-6). IL-6R forms a hexameric complex upon binding 2 Molecules of IL-6 and two molecules of glycoprotein 130 (gp130) which activates intracellular JAK/STAT pathways. Although the normal form of IL-6R is the membrane-bound 80 kDa subunit, a soluble form of IL-6R (sIL-6R) can be generated physiologically by limited proteolysis or alternative splicing. sIL-6R binds to both IL-6 and gp130 generating intracellular signaling. In the immune system, sIL-6R is produced by both na ve and memory CD4 T-cells and strongly augments IL-6 ligand's induction of Th-17 cells.Recombinant human Soluble IL-6 Receptor Alpha (rhsIL-6R alpha) with an N-terminal His tag produced in Sf9 insect cells is a single glycosylated polypeptide chain containing 350 amino acids. A fully biologically active molecule, rhsIL-6R alpha has a molecular mass of 50 kDa analyzed by reducing SDS-PAGE.
Synonyms: gp80 - Molecular Weight
- 50 kDa, observed by reducing SDS-PAGE.
- UniProt
- P08887
- Pathways
- JAK-STAT Signaling, Autophagy, Growth Factor Binding, Cancer Immune Checkpoints
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