CCL7 Protein

Catalog No. ABIN2018233

Product Details

Target: CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: Escherichia coli (E. coli)

Sequence: QPDGTNSSTC CYVKKQKIPK RNLKSYRKIT SSRCPWEAVI FKTKKGMEVC AEAHQKWVEE AIAYLDMKTS TPKP

Characteristics: Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/mL.

Purity: > 95 % by SDS-PAGE and HPLC analyses.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1 EU/μg of rRtMCP-3/CCL7 as determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Buffer: Lyophilized from a 0.2 μm filtered concentrated solution in 2 x PBS, pH 7.4.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C.

Target Details

Target: CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Alternative Name: MCP-3/CCL7 (CCL7 Products)

Synonyms: CCL7 Protein, FIC Protein, MARC Protein, MCP-3 Protein, MCP3 Protein, NC28 Protein, SCYA6 Protein, SCYA7 Protein, Scya7 Protein, fic Protein, marc Protein, mcp3 Protein, C-C motif chemokine ligand 7 Protein, chemokine (C-C motif) ligand 7 Protein, CCL7 Protein, Ccl7 Protein

Background: Monocyte Chemotactic Protein-3 (MCP3) and CCL7 are two monocyte chemotactic proteins produced by human MG63 osteosarcoma cells. Both MCP3 and CCL7 are members of the CC family of chemokines and share 62 % and 71 % amino acid sequence identity, respectively, with MCP1. CCL7 also shares 58 % amino acid identity with MCP3.Similarly to other CC chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.
Synonyms: C-C motif chemokine 7, Monocyte chemoattractant protein 3, Monocyte chemotactic protein 3, MCP-3, Small-inducible cytokine A7, Ccl7, Mcp3, Scya7.

Molecular Weight: 8.5 kDa, a single non-glycosylated polypeptide chain containing 74 amino acids.