CCL8 Protein (AA 24-99)

Catalog No. ABIN2018230

Product Details

Target: CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 24-99

Origin: Human

Source: Escherichia coli (E. coli)

Characteristics: ED50< 0.5 xg/mL, measured by the FLIPR assay using CHO cells transfected with human CCR5, the receptor of human CCL8, corresponding to a specific activity of > 2 x 10^3 units/mg.

Purity: > 95 % by SDS-PAGE analysis.

Endotoxin Level: < 0.2 EU/μg, determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Storage: -80 °C

Storage Comment: Lyophilized recombinant human MCP-2/CCL8(rhMCP-2) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhMCP-2 remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.

Expiry Date: 6 months

Target Details

Target: CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))

Alternative Name: MCP-2/CCL8 (CCL8 Products)

Synonyms: HC14 Protein, MCP-2 Protein, MCP2 Protein, SCYA10 Protein, SCYA8 Protein, 1810063B20Rik Protein, AB023418 Protein, Mcp2 Protein, Scya8 Protein, C-C motif chemokine ligand 8 Protein, chemokine (C-C motif) ligand 8 Protein, C-C motif chemokine 8 Protein, monocyte chemoattractant protein-2 precursor Protein, CCL8 Protein, Ccl8 Protein, LOC101119572 Protein, MCP-2 Protein

Background: MCP-2 is a member of the chemokines, a group of 70-80 residue proteins sharing substantial sequence similarity. Within the chemokines, MCP-2 belongs to the CC subfamily, and is a member of the Monocyte Chemoattractant Proteins (MCPs), which includes MCP-1, MCP-2, MCP-3, MCP-4, and MCP-5. MCP-2 shares 60 % homology with MCP-1, and both proteins can undergo reversible dimerization. The main receptors of MCP-2 are G-protein coupled receptors CCR1 and CCR5. MCP-2 is a potential target in HIV-1 infected human glial cells as it may play a role in the modulation of viral spread in the brain. Recently, researchers found that mouse MCP-2 is expressed in the skin as a novel agonist of CCR8 and plays a role in eosinophilic inflammation.Recombinant human MCP-2/CCL8(rhMCP-2) produced in E. coli is a single non-glycosylated polypeptide chain containing 76 amino acids. A fully biologically active molecule, rhMCP-2 has a molecular mass of 8.9 kDa analyzed by reducing SDS-PAGE.
Synonyms: Monocyte Chemoattractant Protein-2, HC14, SCYA8

Molecular Weight: 8.9 kDa, observed by reducing SDS-PAGE.

UniProt: P80075