Long R3 Insulin Like Growth Factor-1 (Active) Protein
Origin: Human
Host: Escherichia coli (E. coli)
Recombinant
> 95 % by SDS-PAGE.
Active
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- Target
- Long R3 Insulin Like Growth Factor-1
- Protein Type
- Recombinant
- Biological Activity
- Active
- Origin
- Human
- Source
- Escherichia coli (E. coli)
- Sequence
- MFPAMPLSSL FVNGPRTLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKPA KSA
- Characteristics
- ED50 < 10 ng/mL, measured by the dose-dependantproliferation of CHO cells, corresponding to a specific activity of >1.0x 10^5 units/mg.
- Purity
- > 95 % by SDS-PAGE.
- Endotoxin Level
- < 1 EU/μg, determined by LAL method.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstituted in 10 mM HCl at 1 mg/mL. Use a 0.22 μm membrane for filter Sterilization.
- Buffer
- Lyophilized after dissolved in 48 % acetonitrile and 0.1 % TFA
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Lyophilized recombinant human Long R3Insulin-like Growth Factor-1 (rhLR3IGF-1) remains stable up to 6 months at -80 °C from date of receipt. Stock solution of peptide can be stored at least 3 months at -20 °C.
- Expiry Date
- 6 months
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- Target
- Long R3 Insulin Like Growth Factor-1
- Background
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IGF-1 is a well-characterized basic peptide secreted by the liver that circulates in the blood. It has growth-regulating, insulin-like, mitogenic activities. IGF-1 is a growth factor that has a major, but not absolute, dependence on somatotropin. It is believed to be mainly active in adults in contrast to IGF-2, which is also a major fetal growth factor. Human Long R3 Insulin-like Growth Factor-1 (rhLR3IGF-1) contains an 83 amino acid analog of human IGF-I. Compared to the complete human IGF-I sequence, an addition of the rhLR3IGF-1 includes the substitution of an Arg for the Glu at position 3 (hence R3)and a13 amino acid extension peptide at the N-terminus. An enhanced potency is due to the markedly decreased binding of human Long-R3-IGF-I to IGF binding proteins which normally inhibit the biological actions of IGFs. RhLR3IGF-1 is properly folded under oxidizing conditions and then purified by proprietary chromatographic techniques.
Synonyms: Human LR3 Insulin-like Growth Factor-I (LR3-IGF-I) (Receptor Grade) - Molecular Weight
- 9.1±0.9 KDa, observed by reducing SDS-PAGE.
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