IL-8 Protein (AA 28-99, Isoform 1)

Catalog No. ABIN2018161

Product Details

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: Isoform 1, AA 28-99

Origin: Human

Source: Escherichia coli (E. coli)

Characteristics: ED50 < 20 ng/mL, measured by the FLIPR assay using CHO cells transfected with human CXCR1, the receptor of human CXCL8, corresponding to a specific activity of > 5x10^4 units/mg.

Purity: > 95 % as analyzed by SDS-PAGE and HPLC.

Endotoxin Level: < 0.2 EU/μg, determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Storage: -80 °C

Storage Comment: Lyophilized recombinant human Interleukin-8/CXCL8 (rhIL-8) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhIL-8 remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.

Expiry Date: 6 months

Target Details

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Alternative Name: Interleukin-8 (IL-8)/CXCL8 (IL8 Products)

Synonyms: CXCL8 Protein, GCP-1 Protein, GCP1 Protein, LECT Protein, LUCT Protein, LYNAP Protein, MDNCF Protein, MONAP Protein, NAF Protein, NAP-1 Protein, NAP1 Protein, IL-8 Protein, AMCF-I Protein, il8 Protein, IL8 Protein, si:dkey-151b16.2 Protein, cxcli2 Protein, cxcl8 Protein, gcp-1 Protein, il-8 Protein, lynap Protein, mdncf Protein, monap Protein, nap-1 Protein, C-X-C motif chemokine ligand 8 Protein, interleukin 8 Protein, chemokine (C-X-C motif) ligand 8a Protein, chemokine (C-X-C motif) ligand 1-like Protein, chemokine (C-X-C motif) ligand 8 L homeolog Protein, CXCL8 Protein, il8 Protein, cxcl8a Protein, IL8 Protein, Cxcl8 Protein, LOC422654 Protein, cxcl8.L Protein

Background: Interleukin-8 is one of the first discovered chemokines, and belongs to the CXCL family, in which the first two conserved cysteines are separated by one residue. In vivo, IL-8 exists in two forms: 77 a.a. produced by endothelial cells, and the more active 72 a.a. produced by monocytes. The receptors of IL-8 are the seven-helical G-protein coupled receptors CXCR1 and CXCR2, exclusively expressed on neutrophils. The functions of IL-8 are to induce rapid changes in cellular shape, activate the integrins, and release the granule contents of neutrophils. Thus, IL-8 can enhance the antimicrobial actions of defense cells.Recombinant human I Interleukin-8/CXCL8 (rhIL-8) produced in E. coli is a single non-glycosylated polypeptide chain containing 72 amino acids. A fully biologically active molecule, rhIL-8 has a molecular mass of 8.4 kDa analyzed by reducing SDS-PAGE.
Synonyms: CXCL8, monocyte-derived neutrophil chemotactic factor (MDNCF), neutrophil activating factor (NAF), NAP-1

Molecular Weight: 8.4 kDa, observed by reducing SDS-PAGE.

UniProt: P10145

Pathways: TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagy