IL-4 Protein (AA 25-133)

Catalog No. ABIN2018114

Product Details

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 25-133

Origin: Pig

Source: CHO Cells

Characteristics: ED50< 0.25 ng/mL, measured in a cell proliferation assay using TF-1 human erythroleukemic cells, corresponding to a specific activity of >4 x 10^6 units/mg
AA 25-133, expressed with an N-terminal Met.

Purity: > 95 % as analyzed by SDS-PAGE and HPLC.

Endotoxin Level: < 0.2 EU/μg, determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O or PBS at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Storage: -80 °C

Storage Comment: Lyophilized recombinant porcine Interleukin 4 (IL-4) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rpIL-4 should be stable up to 1 week at 4 °C or up to 2 months at -20 °C.

Expiry Date: 6 months

Target Details

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Alternative Name: Interleukin-4 (IL-4) (IL4 Products)

Background: Interleukin-4 (IL-4) is a pleiotropic cytokine regulates diverse T and B cell responses including cell proliferation, survival, and gene expression. It has important effects on the growth and differentiation of different immunologically competent cells. Interleukin-4 is produced by mast cells, T cells, and bone marrow stromal cells [1]. IL-4 regulates the differentiation of native CD4+ T cells (Th0 cells) into helper Th2 cells, and regulates the immunoglobulin class switching to the IgG1 and IgE isotypes. IL-4 has numerous important biological functions including stimulating B-cells activation, T-cell proliferation and CD4+ T-cells differentiation to Th2 cells. It is a key regulator in hormone control and adaptive immunity [2]. IL-4 also plays a major role in inflammation response and wound repair via activation of macrophage into M2 cells [3]. IL-4 is stabilized by three disulphide bonds forming a compact globular protein structure [4]. Four alpha-helix bundle with left-handed twist is dominated half of the protein structure with 2 overhand connections and fall into a 2-stranded anti-parallel beta sheet [5].
Synonyms: Interleukin-4, BCGF, BCDF, B cell stimulating factor, BSF-1, Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra

Molecular Weight: 15 kDa, observed by reducing SDS-PAGE

UniProt: Q04745

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation