CCL14 Protein (AA 22-93)

Catalog No. ABIN2017951

Product Details

Target: CCL14 (Chemokine (C-C Motif) Ligand 14 (CCL14))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 22-93

Origin: Human

Source: Escherichia coli (E. coli)

Characteristics: ED50 < 25 μg/mL, measured by the FLIPR assay using CHO cells transfected with human CCR5, the receptor of human CCL14, corresponding to a specific activity of > 40 units/mg.

Purity: > 95 % by SDS-PAGE analysis.

Endotoxin Level: < 0.2 EU/μg, determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Storage: -80 °C

Storage Comment: Lyophilized recombinant human Hemofiltrate CC Chemokine-1 (72 a.a.) (HCC-1)/CCL14 (rhHCC-1) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhHCC-1 remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.

Expiry Date: 6 months

Target Details

Target: CCL14 (Chemokine (C-C Motif) Ligand 14 (CCL14))

Abstract: CCL14 Products

Synonyms: CCL14 Protein, CC-1 Protein, CC-3 Protein, CKB1 Protein, HCC-1 Protein, HCC-1(1-74) Protein, HCC-1/HCC-3 Protein, HCC-3 Protein, MCIF Protein, NCC-2 Protein, NCC2 Protein, SCYA14 Protein, SCYL2 Protein, SY14 Protein, C-C motif chemokine ligand 14 Protein, C-C motif chemokine 14 Protein, chemokine (C-C motif) ligand 14 Protein, CCL14 Protein, Ccl14 Protein, LOC480603 Protein, LOC101115044 Protein

Background: HCC-1/CCL14 is a member of the chemokine family, which are small chemotactic proteins that regulate cell migration under inflammatory and steady state conditions. HCC-1 is expressed in epithelial and decidual cells and is unique among chemokines due to its high abundance in normal human plasma. HCC-1 can bind to chemokine receptors CCR1 and CCR5, however full length HCC-1 is a weak agonist of CCR1 and only becomes potent after removal of its eight N-terminal residues. Chemokine decoy receptor D6 can bind HCC-1 and promote its degradation as a means to regulate its level in vivo. Functionally HCC-1 promotes trophoblast migration by regulating extracellular matrix components as well as specific adhesion molecules.Recombinant human Hemofiltrate CC Chemokine-1 (72 a.a.) (HCC-1)/CCL14 (rhHCC-1) produced in E. coli is a single non-glycosylated polypeptide chain containing 72 amino acids. A fully biologically active molecule, rhHCC-1 has a molecular mass of 8.4 kDa analyzed by reducing SDS-PAGE.
Synonyms: NCC-2, SCYA14

Molecular Weight: 8.4 kDa, observed by reducing SDS-PAGE.

UniProt: Q16627