FGF2 Protein (AA 10-154)

Catalog No. ABIN2017861

Product Details

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 10-154

Origin: Rat

Source: Escherichia coli (E. coli)

Characteristics: ED50 < 0.25 ng/mL, measured by a cell proliferation assay using 3T3 cells, corresponding to a specific activity of > 4 x 10^6 units/mg.
AA 10-154, expressed with an N-terminal Gly.

Purity: > 95 % by SDS-PAGE analysis.

Endotoxin Level: < 0.2 EU/μg, determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Storage: -80 °C

Storage Comment: Lyophilized recombinant rat Fibroblast Growth Factor-basic (rrFGF-basic) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rrFGF-basic remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.

Expiry Date: 6 months

Target Details

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Alternative Name: Fibroblast Growth Factor-Basic (FGF-Basic) (FGF2 Products)

Synonyms: BFGF Protein, FGF-2 Protein, FGFB Protein, HBGF-2 Protein, Fgf-2 Protein, Fgfb Protein, bFGF Protein, fibroblast growth factor 2 Protein, fibroblast growth factor 2 (basic) Protein, FGF2 Protein, Fgf2 Protein, fgf2 Protein

Background: Fibroblast Growth Factor-basic (FGF-basic), also known as FGF-2, is a pleiotropic cytokine and one of the prototypic members of the heparin-binding FGF family. Like other FGF family members, FGF-basic has the beta trefoil structure. In vivo, FGF-basic is produced by a variety of cells, including cardiomycotes, fibroblasts, and vascular cells. FGF-basic regulates a variety of processes including cell proliferation, differentiation, survival, adhesion, motility, apoptosis, limb formation and wound healing. FGF-basic can be tumorigenic due to its role in angiogenesis and blood vessel remodeling. The angiogenic effects of FGF-basic can produce beneficial cardioprotection during acute heart injury.Recombinant rat Fibroblast Growth Factor-basic (rrFGF-basic) produced in E. coli is a single non-glycosylated polypeptide chain containing 146 amino acids. A fully biologically active molecule, rrFGF-basic has a molecular mass of 16.4 kDa analyzed by reducing SDS-PAGE.
Synonyms: Fibroblast Growth Factor-basic, FGF-2, HBGF-2, Prostatropin

Molecular Weight: 16.4 kDa, observed by reducing SDS-PAGE.

UniProt: P13109

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteins