FGF9 Protein (AA 3-208, Ser34Asn-Mutant)

Catalog No. ABIN2017840

Product Details

Target: FGF9 (FGF-9) (Fibroblast Growth Factor 9 (FGF-9))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 3-208, Ser34Asn-Mutant

Origin: Mouse

Source: Escherichia coli (E. coli)

Characteristics: ED50 < 5 ng/mL, measured by a cell proliferation assay using 3T3 cells, corresponding to a specific activity of > 2x10^5 units/mg.
AA 3-208 (Ser34Asn), expressed with an N-terminal Met.

Purity: > 95 % as analyzed by SDS-PAGE and HPLC.

Endotoxin Level: < 0.2 EU/μg, determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Storage: -80 °C

Storage Comment: Lyophilized recombinant mouse Fibroblast Growth Factor (rmFGF-9) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rmFGF-9 remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.

Expiry Date: 6 months

Target Details

Target: FGF9 (FGF-9) (Fibroblast Growth Factor 9 (FGF-9))

Alternative Name: Fibroblast Growth Factor-9 (FGF-9) (FGF-9 Products)

Synonyms: Eks Protein, FGF-9 Protein, GAF Protein, HBFG-9 Protein, HBGF-9 Protein, SYNS3 Protein, XFGF-9 Protein, fgf9-A Protein, fibroblast growth factor 9 Protein, fibroblast growth factor 9 S homeolog Protein, Fgf9 Protein, FGF9 Protein, fgf9.S Protein

Background: Fibroblast Growth Factor-9 (FGF-9) is a pleiotropic cytokine and belongs to the heparin-binding FGF family. Like other members in the family, FGF-9 resembles a beta-trefoil structure. FGF-9 undergoes reversible dimerization, a common characteristic shared by its subfamily members, FGF-16 and FGF-20. The mutations involved in the homodimerization also affect the affinity for heparin, binding to FGF receptors, and biological activity. In vivo, FGF-9 is expressed in limb buds, the developing skeleton, and in the intestines during late stage embryogenesis. FGF-9 is essential for the development of heart, lung, kidney, cecum, and testes, and the reduction of FGF-9 level leads to premature differentiation. FGF-9 also works along with Bone Morphogenetic Protein-7 (BMP-7) to promote the survival of nephron progenitors.Recombinant mouse Fibroblast Growth Factor (rmFGF-9) produced in E. coli is a single non-glycosylated polypeptide chain containing 207 amino acids. A fully biologically active molecule, rmFGF-9 has a molecular mass of 23.4 kDa analyzed by reducing SDS-PAGE.
Synonyms: Growth Factor-9, GAF (Glia-activating factor), HBGF-9

Molecular Weight: 23.4 kDa, observed by reducing SDS-PAGE.

UniProt: P54130