FGF8 Protein (AA 23-215)

Catalog No. ABIN2017830

Product Details

Target: FGF8 (Fibroblast Growth Factor 8 (Androgen-Induced) (FGF8))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 23-215

Origin: Human

Source: Escherichia coli (E. coli)

Characteristics: ED50 < 5.0 ng/mL, measured by a cell proliferation assay using 3T3 cells in the presence of 1 μg/mL of heparin, corresponding to a specific activity of > 2.0x 10^5 units/mg.
AA 23-215, expressed with an N-terminal Met.

Purity: > 95 % by SDS-PAGE and HPLC analyses.

Endotoxin Level: < 0.2 EU/μg, determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Storage: -80 °C

Storage Comment: Lyophilized recombinant human Fibroblast Growth Factor-8 (rhFGF-8) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhFGF-8 should be stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.

Expiry Date: 6 months

Target Details

Target: FGF8 (Fibroblast Growth Factor 8 (Androgen-Induced) (FGF8))

Alternative Name: Fibroblast Growth Factor-8 (FGF-8) (FGF8 Products)

Synonyms: Aigf Protein, Fgf-8 Protein, AIGF Protein, FGF-8 Protein, HBGF-8 Protein, HH6 Protein, KAL6 Protein, fibroblast growth factor 8 Protein, Fgf8 Protein, FGF8 Protein

Background: Fibroblast Growth Factor-8 (FGF-8) is a heparin-binding growth factor of the FGF family. There are 4 known forms of FGF8 produced by alternative splicing: FGF8a, FGF-8b, FGF-8e and FGF-8f. The human and mouse FGF8b are identical of aa sequences. FGF-8 plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. FGF-8 is required for normal brain, eye, ear and limb development during embryogenesis. It is also required for normal development of the gonadotropin- releasing hormone (GnRH) neuronal system.Recombinant human Fibroblast Growth Factor-8 (rhFGF-8) produced in E. coli is a single non-glycosylated polypeptide chain containing 194 amino acids. A fully biologically active molecule, rhFGF-8 has a molecular mass of 22.5 kDa analyzed by reducing SDS-PAGE.
Synonyms: FGF-8b, AIGF, HBGF

Molecular Weight: 22.5kDa, observed by reducing SDS-PAGE.

UniProt: P55075

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis