CCL11 Protein

Catalog No. ABIN7477969

Product Details

Target: CCL11 (Chemokine (C-C Motif) Ligand 11 (CCL11))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Sequence: HPGSIPTSCC FIMTSKKIPN TLLKSYKRIT NNRCTLKAIV FKTRLGKEIC ADPKKKWVQD ATKHLDQKLQ TPKP

Characteristics: Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using purified eosinophils is in a concentration range of 100-1000 ng/mL.

Purity: > 96 % by SDS-PAGE and HPLC analyses.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1 EU/μg of rMuEotaxin/CCL11 as determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Buffer: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C.

Target Details

Target: CCL11 (Chemokine (C-C Motif) Ligand 11 (CCL11))

Alternative Name: Eotaxin/CCL11 (CCL11 Products)

Synonyms: SCYA11 Protein, Scya11 Protein, eotaxin Protein, Eotaxin Protein, C-C motif chemokine ligand 11 Protein, chemokine (C-C motif) ligand 11 Protein, CCL11 Protein, Ccl11 Protein

Background: Eotaxin also called CCL11 is a CC chemokine that signals through the CCR3 receptor. It is produced by IFN-gamma stimulated endothelial cells and TNF-activated monocytes. Eotaxin selectively chemoattracts eosinophils and along Eotaxin-2 and Eotaxin-3, plays a key role in the regulation of eosinophil recruitment in the asthmatic lung, and in allergic reactions.
Synonyms: Eotaxin murine, CCL11 murine

Molecular Weight: 8.4 kDa, a single non-glycosylated polypeptide chain containing 74 amino acids.

Pathways: Regulation of Actin Filament Polymerization, The Global Phosphorylation Landscape of SARS-CoV-2 Infection