Defensin beta 4 Protein (DEFB4)

Catalog No. ABIN2017684

Product Details

Target: Defensin beta 4 (DEFB4)

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: Escherichia coli (E. coli)

Sequence: QSINNPITCL TKGGVCWGPC TGGFRQIGTC GLPRVRCCKK K

Characteristics: Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 0.1-100.0 ng/mL.

Purity: > 95 % by SDS-PAGE and HPLC analyses.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1 EU/μg of rRtBD-4 as determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Buffer: Lyophilized from a 0.2 μm filtered concentrated solution in 10 mM PB, pH 7.4, 500 mM NaCl.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C.

Target Details

Target: Defensin beta 4 (DEFB4)

Alternative Name: beta-Defensin 4 (BD-4) (DEFB4 Products)

Synonyms: BD-2 Protein, DEFB-2 Protein, DEFB102 Protein, DEFB2 Protein, DEFB4 Protein, HBD-2 Protein, SAP1 Protein, 2310001F05Rik Protein, BD-4 Protein, Defb2 Protein, Defb3 Protein, BNBD-4 Protein, BNBD4 Protein, defensin beta 4A Protein, defensin beta 4 Protein, defensin, beta 4A Protein, DEFB4A Protein, Defb4 Protein

Background: Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The a-defensins are distinguished from the b-defensins by the pairing of their three disulfide bonds. To date, four rat b-defensins have been identified, BD-1, BD-2, BD-3 and BD-4. The b-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence. b-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Beta-defensins are 3-5 kDa peptides ranging in size from 33-47 amino acid residues. BD-4 is expressed in testis, stomach, uterus, neutrophils, thyroid, lung and kidney.
Synonyms: Beta-defensin 4, BD-4, BD-2, Defensin beta 4, RBD-2, RBD-4, Defb4, Defb2, Defb3.

Molecular Weight: 4.4 kDa, a single non-glycosylated polypeptide chain containing 41 amino acids.