beta Defensin 1 Protein

Catalog No. ABIN2017678

Product Details

Target: beta Defensin 1 (DEFB1) (Defensin, beta 1 (DEFB1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: Escherichia coli (E. coli)

Sequence: DQYRCLQNGG FCLRSSCPSH TKLQGTCKPD KPNCCRS

Characteristics: Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using CD34+ dendritic cells is in a concentration range of 100.0-1000.0 ng/mL.

Purity: > 95 % by SDS-PAGE and HPLC analyses.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1 EU/μg of rRtBD-1 as determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Buffer: Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, 500 mM NaCl, pH 7.0.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C.

Target Details

Target: beta Defensin 1 (DEFB1) (Defensin, beta 1 (DEFB1))

Alternative Name: beta-Defensin 1(BD-1) (DEFB1 Products)

Synonyms: BD1 Protein, DEFB-1 Protein, DEFB101 Protein, HBD1 Protein, AW260221 Protein, BD-1 Protein, DEFB1-like Protein, RHBD-1 Protein, CBD1 Protein, PBD-1 Protein, DEFB1 Protein, BNBD-1 Protein, defensin beta 1 Protein, avian beta-defensin 1 Protein, defensin, beta 1 Protein, beta-defensin 1-like Protein, beta-defensin 1 Protein, DEFB1 Protein, Defb1 Protein, AvBD1 Protein, LOC100861170 Protein

Background: Defensin are 3-4 kDa antimicrobial peptides of which three distinct families have been identified, alpha-defensin, beta-defensins, and insect defensins. Rat beta-Defensin 1, as a member of the beta-defensin family, is a salt-sensitive antimicrobial peptide present in epithelia of the lung and urogenital tract. The predicted amino acid sequence shows the hallmark features of other known epithelial beta-defensins, including the ordered array of six cysteine residues.
Synonyms: Beta-defensin 1, BD-1, rBD-1, Defensin beta 1, Defb1.

Molecular Weight: 4.1 kDa, a single non-glycosylated polypeptide chain containing 37 amino acids.