DEFB103B Protein
DEFB103B
Origin: Mouse
Host: Escherichia coli (E. coli)
Recombinant
> 95 % by SDS-PAGE and HPLC analyses.
Active
-
- Target
- DEFB103B (Defensin, beta 103B (DEFB103B))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Origin
- Mouse
- Source
- Escherichia coli (E. coli)
- Sequence
- KKINNPVSCL RKGGRCWNRC IGNTRQIGSC GVPFLKCCKR K
- Characteristics
- Fully biologically active when compared to standard. The ED50 as determined by anti-microbial activity against E.coli. is less than 20 μg/mL, corresponding to a specific activity of > 50 IU/mg.
- Purity
- > 95 % by SDS-PAGE and HPLC analyses.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- < 1 EU/μg of rMuBD-3 as determined by LAL method.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
- Buffer
- Lyophilized from a 0.2 μm filtered concentrated solution in 2 x PBS, pH 7.4.
- Handling Advice
- Avoid repeated freeze/thaw cycles.
- Storage
- 4 °C/-20 °C
- Storage Comment
- This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C.
-
- Target
- DEFB103B (Defensin, beta 103B (DEFB103B))
- Alternative Name
- beta Defensin-3(BD-3)
- Synonyms
- BD-3 Protein, DEFB103 Protein, DEFB3 Protein, HBD3 Protein, HBP3 Protein, defensin beta 103B Protein, DEFB103B Protein
- Background
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Beta defensin-3, also known as BD-3 and DEFB-3, is a membrane active cationic peptide that functions in inflammation and innate immune responses and coded by Defb 3 gene on chromosome 8 in mouse. There are at least 30 beta-defensins which are distinguished from beta-defensins by the connectivity pattern of their three intramolecular disulfide bonds. BD3 is widely expressed among epithelial tissues, notably by keratinocytes and airway epithelial cells. It is upregulated in response to proinflammatory cytokines, microbial and viral infections, and at the edges of skin wounds. BD3 induction in osteoarthritis chondrocytes promotes MMP1 and 13 productions and inhibits TIMP1 and 2 expressions.
Synonyms: BD-3 Murine, BD 3 Murine - Molecular Weight
- 4.6 kDa, a single non-glycosylated polypeptide chain containing 41 amino acids.
- Pathways
- Production of Molecular Mediator of Immune Response
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