CX3CL1 Protein (AA 25-337) (His tag)

Catalog No. ABIN1945442

Product Details

Target: CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))

Protein Type: Recombinant

Protein Characteristics: AA 25-337

Origin: Mouse

Source: Human Cells

Purification tag / Conjugate: This CX3CL1 protein is labelled with His tag.

Purpose: Recombinant Mouse C-X3-C Motif Chemokine 1/CX3CL1/Fractalkine (C-6His)

Sequence: QHLGMTKCEI MCDKMTSRIP VALLIRYQLN QESCGKRAIV LETTQHRRFC ADPKEKWVQD AMKHLDHQAA ALTKNGGKFE KRVDNVTPGI TLATRGLSPS ALTKPESATL EDLALELTTI SQEARGTMGT SQEPPAAVTG SSLSTSEAQD AGLTAKPQSI GSFEAADIST TVWPSPAVYQ SGSSSWAEEK ATESPSTTAP SPQVSTTSPS TPEENVGSEG QPPWVQGQDL SPEKSLGSEE INPVHTDNFQ ERGPGNTVHP SVAPISSEET PSPELVASGS QAPKIEEPIH ATADPQKLSV LITPVPDTQA ATRVDHHHHH H

Characteristics: Recombinant Mouse Fractalkine/C-X3-C motif chemokine 1/CX3C membrane-anchored chemokine/Neurotactin/Small-inducible cytokine D1 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (AA 25-337) of Human CX3CL1 fused with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))

Alternative Name: CX3CL1 (CX3CL1 Products)

Synonyms: ABCD3 Protein, CX3CL1 Protein, DKFZp459K117 Protein, Cx3c Protein, Scyd1 Protein, ABCD-3 Protein, C3Xkine Protein, CXC3 Protein, CXC3C Protein, NTN Protein, NTT Protein, SCYD1 Protein, fractalkine Protein, neurotactin Protein, AB030188 Protein, AI848747 Protein, CX3C Protein, Cxc3 Protein, D8Bwg0439e Protein, ATP binding cassette subfamily D member 3 Protein, C-X3-C motif chemokine ligand 1 Protein, chemokine (C-X3-C motif) ligand 1 Protein, ABCD3 Protein, CX3CL1 Protein, Cx3cl1 Protein, Abcd3 Protein

Background: Fractalkine(CX3CL1) is a single-pass type I membrane protein and belongs to the intercrine delta family. It consists of an extracellular NH2-terminal domain, a mucin-like stalk, a transmembrane α helix, and a short cytoplasmic tail. CX3CL1 exists in two forms: as a membrane-anchored or as a shed 80-95K glycoprotein. Soluble CX3CL1 is generated by limited proteolysis on the cell surface, and a disintegrin and metallopeptidase 10 (ADAM10) and ADAM17/tumor necrosis factor-α-converting enzyme (ADAM17/TACE) participate in this shedding. It has been suggested that ADAM10 acts in the constitutive shedding, and ADAM17 acts in response to cell activation. The protein may play a role in regulating leukocyte adhesion and migration processes at the endothelium.

Molecular Weight: 34.3 kDa

UniProt: O35188

Pathways: Synaptic Membrane