EIF4E Protein (AA 1-217)

Catalog No. ABIN1945364

Product Details

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Protein Type: Recombinant

Protein Characteristics: AA 1-217

Origin: Human

Source: Escherichia coli (E. coli)

Purpose: Recombinant Human Eukaryotic Translation Initiation Factor 4E/EIF4E

Sequence: MATVEPETTP TPNPPTTEEE KTESNQEVAN PEHYIKHPLQ NRWALWFFKN DKSKTWQANL RLISKFDTVE DFWALYNHIQ LSSNLMPGCD YSLFKDGIEP MWEDEKNKRG GRWLITLNKQ QRRSDLDRFW LETLLCLIGE SFDDYSDDVC GAVVNVRAKG DKIAIWTTEC ENREAVTHIG RVYKERLGLP PKIVIGYQSH ADTATKSGST TKNRFVV

Characteristics: Recombinant Human eIF-4E is produced by our E. coli expression system. The target protein is expressed with sequence (AA 1-217) of Human eIF-4E .

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Alternative Name: Eukaryotic Translation Initiation Factor 4E (EIF4E Products)

Synonyms: AUTS19 Protein, CBP Protein, EIF4E1 Protein, EIF4EL1 Protein, EIF4F Protein, eif4e-1 Protein, zgc:86680 Protein, EG668879 Protein, Eif4e-ps Protein, If4e Protein, eIF-4E Protein, 0260/09 Protein, 0587/11 Protein, 0589/11 Protein, 0919/12 Protein, 1004/13 Protein, 2 Protein, 7238 Protein, CG4035 Protein, CT13384 Protein, CT39424 Protein, CT39426 Protein, D-eIF4E Protein, Dmel\\CG4035 Protein, Eif4E Protein, Eif4e Protein, IF4E Protein, dEif4e Protein, deIF-4E Protein, deIF4E Protein, eIF 4E Protein, eIF-4E1 Protein, eIF-4E2 Protein, eIF-4EII Protein, eIF-4e Protein, eIF4E Protein, eIF4E-1 Protein, eIF4E-1/2 Protein, eIF4E-2 Protein, eIF4E1 Protein, eIF4EI Protein, eIF4e Protein, eif-4E Protein, eif-4e Protein, eif4e Protein, l(3)07238 Protein, l(3)67Af Protein, l(3)S025007 Protein, l(3)S026009 Protein, l(3)S058711 Protein, l(3)S091912 Protein, cbp Protein, eif4f Protein, eif4e1 Protein, eif4el1 Protein, EIF-4E Protein, zgc:101581 Protein, eukaryotic translation initiation factor 4E Protein, eukaryotic translation initiation factor 4ea Protein, Eukaryotic initiation factor 4E Protein, eucaryotic initiation factor 6 Protein, eukaryotic translation initiation factor 4E L homeolog Protein, eukaryotic translation initiation factor 4E family member 1B S homeolog Protein, translation initiation factor eIF4E Protein, eukaryotic translation initiation factor 4eb Protein, EIF4E Protein, eif4ea Protein, Eif4e Protein, eIF-4E Protein, eif6 Protein, eif4e Protein, eif4e.L Protein, eif4e1b.S Protein, LOC100533326 Protein, eif4eb Protein

Background: Eukaryotic translation initiation factor 4E is a 217 amino acids protein that belongs to the eukaryotic initiation factor 4E family. eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners.It can recognize and bind the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.

Molecular Weight: 25.1 kDa

UniProt: P06730

Pathways: BCR Signaling