CKM Protein (AA 1-381) (His tag)
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- Target See all CKM Proteins
- CKM (Creatine Kinase, Muscle (CKM))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-381
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Origin
- Human
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Source
- Human Cells
- Purification tag / Conjugate
- This CKM protein is labelled with His tag.
- Purpose
- Recombinant Human Creatine Kinase, Muscle/CKMM (C-6His)
- Sequence
- MPFGNTHNKF KLNYKPEEEY PDLSKHNNHM AKVLTLELYK KLRDKETPSG FTVDDVIQTG VDNPGHPFIM TVGCVAGDEE SYEVFKELFD PIISDRHGGY KPTDKHKTDL NHENLKGGDD LDPNYVLSSR VRTGRSIKGY TLPPHCSRGE RRAVEKLSVE ALNSLTGEFK GKYYPLKSMT EKEQQQLIDD HFLFDKPVSP LLLASGMARD WPDARGIWHN DNKSLLVWVN EEDHLRVISM EKGGNMKEVF RRFCVGLQKI EEIFKKAGHP FMWNQHLGYV LTCPSNLGTG LRGGVHVKLA HLSKHPKFEE ILTRLRLQKR GTGGVDTAAV GSVFDVSNAD RLGSSEVEQV QLVVDGVKLM VEMEKKLEKG QSIDDMIPAQ KVDHHHHHH
- Characteristics
- Recombinant Human Creatine Kinase, Muscle/CKMM is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Met1-Lys381) of Human CKMM fused with a polyhistidine tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product CKM Protein
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl,150 mM NaCl,10 % Glycerol, pH 7.5.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- CKM (Creatine Kinase, Muscle (CKM))
- Alternative Name
- CKMM (CKM Products)
- Synonyms
- CKMM Protein, M-CK Protein, Ckmm Protein, MCK Protein, ckmm Protein, m-ck Protein, CKM Protein, CK-M Protein, cb51 Protein, ckm Protein, mck Protein, wu:fa28d05 Protein, ckm3 Protein, wu:fb55e09 Protein, zgc:64204 Protein, zgc:92070 Protein, creatine kinase, M-type Protein, creatine kinase, muscle Protein, creatine kinase, M-type L homeolog Protein, creatine kinase, muscle a Protein, creatine kinase, muscle b Protein, CKM Protein, Ckm Protein, ckm.L Protein, ckm Protein, ckma Protein, ckmb Protein
- Sub Type
- Fusionprotein
- Background
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Creatine kinase M-type is also known as Creatine kinase M chain,M-CK. It is a protein that in humans is encoded by the CKM gene. It belongs to the ATP:guanido phosphotransferase family,containing 1 phosphagen kinase C-terminal domain and 1 phosphagen kinase N-terminal domain. Creatine kinase M-type can reversibly catalyzes the transfer of phosphate between ATP and various phosphogens. It plays a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Alternative Names: Creatine kinase M-type is also known as Creatine kinase M chain,M-CK. - Molecular Weight
- 44.1 kDa
- UniProt
- P06732
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