BPI Protein (AA 32-487) (His tag)
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- Target See all BPI Proteins
- BPI (Bactericidal/Permeability Increasing Protein (BPI))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 32-487
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Origin
- Human
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Source
- Human Cells
- Purification tag / Conjugate
- This BPI protein is labelled with His tag.
- Purpose
- Recombinant Human Bactericidal Permeability-Increasing Protein/BPI/CAP57 (C-6His)
- Sequence
- VNPGVVVRIS QKGLDYASQQ GTAALQKELK RIKIPDYSDS FKIKHLGKGH YSFYSMDIRE FQLPSSQISM VPNVGLKFSI SNANIKISGK WKAQKRFLKM SGNFDLSIEG MSISADLKLG SNPTSGKPTI TCSSCSSHIN SVHVHISKSK VGWLIQLFHK KIESALRNKM NSQVCEKVTN SVSSKLQPYF QTLPVMTKID SVAGINYGLV APPATTAETL DVQMKGEFYS ENHHNPPPFA PPVMEFPAAH DRMVYLGLSD YFFNTAGLVY QEAGVLKMTL RDDMIPKESK FRLTTKFFGT FLPEVAKKFP NMKIQIHVSA STPPHLSVQP TGLTFYPAVD VQAFAVLPNS SLASLFLIGM HTTGSMEVSA ESNRLVGELK LDRLLLELKH SNIGPFPVEL LQDIMNYIVP ILVLPRVNEK LQKGFPLPTP ARVQLYNVVL QPHQNFLLFG ADVVYKVDHH HHHH
- Characteristics
- Recombinant Human Bactericidal permeability-increasing protein/BPI/CAP57 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Val32-Lys487) of Human BPI fused with a polyhistidine tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- 4 °C/-20 °C/-80 °C
- Storage Comment
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - Expiry Date
- 3 months
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- Target
- BPI (Bactericidal/Permeability Increasing Protein (BPI))
- Alternative Name
- BPI (BPI Products)
- Synonyms
- 9230105K17Rik Protein, Bpifd1 Protein, BPIFD1 Protein, rBPI Protein, bpi Protein, bpi.L Protein, lbp Protein, bactericidal/permeability-increasing protein Protein, bactericidal permeablility increasing protein Protein, bactericidal/permeability-increasing protein S homeolog Protein, BPI Protein, Bpi Protein, bpi.S Protein
- Sub Type
- Fusionprotein
- Background
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Bactericidal permeability-increasing protein(BPI for short), is a secreted protein which belongs to the BPI/LBP/Plunc superfamily, BPI/LBP family. It exists as a monomer or a disulfide-linked homodimer. The cytotoxic action of BPI is limited to many species of Gram-negative bacteria. This specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. BPI has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, and this activity is inhibited by LPS from P.aeruginosa.
Alternative Names: Bactericidal permeability-increasing protein(BPI for short), is a secreted protein which belongs to the BPI/LBP/Plunc superfamily, BPI/LBP family. - Molecular Weight
- 52.5 kDa
- UniProt
- P17213
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