PARP1 Protein

Catalog No. ABIN1741729

Product Details

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus

Application: Western Blotting (WB), ELISA, Poly ADP ribose research (PAR)

Specificity: Biological Activity: 20000 U/mg PARP1,Protein targets

Characteristics: PARP1 Enzyme is highly purified and enzymatically active human PARP1, expressed in a baculovirus expression system. It is useful for high-throughput enzymatic assays, visualization of the automodification reaction by SDS-PAGE and Western blotting, ELISA, standard for SDS-PAGE and WB, and in other assays.
Full-length hPARP1

Purification: Affinity chromatography

Purity: > 95 %

Application Details

Application Notes: 10 ng/reaction

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconsitute in dH2O

Storage: -20 °C

References

Cinque, Ferino, Pedersen, Xodo: "Role of Poly [ADP-ribose] Polymerase 1 in Activating the Kirstenras (KRAS) Gene in Response to Oxidative Stress." in: International journal of molecular sciences, Vol. 21, Issue 17, (2020) (PubMed).

Zhang, Wang, Ding, Yu: "Site-specific characterization of the Asp- and Glu-ADP-ribosylated proteome." in: Nature methods, Vol. 10, Issue 10, pp. 981-4, (2013) (PubMed).

Target Details

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Alternative Name: Poly ADP ribose Polymerase 1 (PARP1 Products)

Background: Poly (ADP-ribose) polymerase-1 (PARP1) is an abundant and ubiquitous nuclear enzyme that catalyzes the NAD(+)-dependent addition of ADP-ribose polymers on a variety of nuclear proteins.

Molecular Weight: 113 kDa

Pathways: Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location