LMCD1 Protein (AA 1-365) (His tag)
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- Target See all LMCD1 Proteins
- LMCD1 (LIM and Cysteine-Rich Domains 1 (LMCD1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-365
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This LMCD1 protein is labelled with His tag.
- Purpose
- Recombinant Human LIM and Cysteine-Rich Domains Protein 1/LMCD1/Dyxin (N, C-6His)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MAKVAKDLNP GVKKMSLGQL QSARGVACLG CKGTCSGFEP HSWRKICKSC KCSQEDHCLT SDLEDDRKIG RLLMDSKYST LTARVKGGDG IRIYKRNRMI MTNPIATGKD PTFDTITYEW APPGVTQKLG LQYMELIPKE KQPVTGTEGA FYRRRQLMHQ LPIYDQDPSR CRGLLENELK LMEEFVKQYK SEALGVGEVA LPGQGGLPKE EGKQQEKPEG AETTAATTNG SLSDPSKEVE YVCELCKGAA PPDSPVVYSD RAGYNKQWHP TCFVCAKCSE PLVDLIYFWK DGAPWCGRHY CESLRPRCSG CDEIIFAEDY QRVEDLAWHR KHFVCEGCEQ LLSGRAYIVT KGQLLCPTCS KSKRSLEHHH HHH
- Characteristics
- Recombinant Human LIM and cysteine-rich domains protein 1/LMCD1/Dyxin is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Ser365) of Human LMCD1 fused with a 6His tag at the N-terminus and a 6His tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product LMCD1 Protein
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- LMCD1 (LIM and Cysteine-Rich Domains 1 (LMCD1))
- Alternative Name
- LMCD1 (LMCD1 Products)
- Synonyms
- LMCD1 Protein, AW455500 Protein, AW553074 Protein, dyxin Protein, LIM and cysteine rich domains 1 Protein, LIM and cysteine-rich domains 1 Protein, LMCD1 Protein, lmcd1 Protein, Lmcd1 Protein
- Sub Type
- Fusionprotein
- Background
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LMCD1 is transcriptional cofactor which contains a cysteine-rich domain in the N-terminal region and 2 LIM domains in the C-terminal region. It also has several potential phosphorylation and N-myristoylation sites and a single potential N-glycosylation site. LMCD1 is expressed in many tissues with highest abundance in skeletal muscle. LMCD1 restricts GATA6 function by inhibiting DNA-binding, resulting in repression of GATA6 transcriptional activation of downstream target genes. It plays a critical role in the development of cardiac hypertrophy via activation of calcineurin/nuclear factor of activated T-cells signaling pathway.
Alternative Names: LIM and cysteine-rich domains protein 1, LMCD1, Dyxin - Molecular Weight
- 44 kDa
- UniProt
- Q9NZU5
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