UBE2K Protein (AA 1-200) (His tag,SUMO Tag)
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- Target See all UBE2K Proteins
- UBE2K (Ubiquitin-Conjugating Enzyme E2K (UBE2K))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-200
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This UBE2K protein is labelled with His tag,SUMO Tag.
- Purpose
- Recombinant Human Ubiquitin-Conjugating Enzyme E2 K/UBE2K (N-6His, SUMO tag)
- Sequence
- MGHHHHHHGS DSEVNQEAKP EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQAPEDLD MEDNDIIEAH REQIGGMANI AVQRIKREFK EVLKSEETSK NQIKVDLVDE NFTELRGEIA GPPDTPYEGG RYQLEIKIPE TYPFNPPKVR FITKIWHPNI SSVTGAICLD ILKDQWAAAM TLRTVLLSLQ ALLAAAEPDD PQDAVVANQY KQNPEMFKQT ARLWAHVYAG APVSSPEYTK KIENLCAMGF DRNAVIVALS SKSWDVETAT ELLLSN
- Characteristics
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Recombinant Human Ubiquitin-Conjugating Enzyme E2 K/UBE2K (N-6His, SUMO tag)
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product UBE2K Protein
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- UBE2K (Ubiquitin-Conjugating Enzyme E2K (UBE2K))
- Alternative Name
- Ubiquitin-Conjugating Enzyme E2 K/UBE2K (UBE2K Products)
- Synonyms
- E2-25K Protein, HIP2 Protein, HYPG Protein, LIG Protein, UBC1 Protein, E2-25k Protein, Hip2 Protein, Hypg Protein, Lig Protein, e2-25k Protein, hip2 Protein, hypg Protein, lig Protein, AW492011 Protein, D5Ertd601e Protein, ube2k Protein, zgc:103472 Protein, zgc:110791 Protein, ubiquitin conjugating enzyme E2 K Protein, ubiquitin-conjugating enzyme E2K Protein, ubiquitin conjugating enzyme E2 K S homeolog Protein, ubiquitin-conjugating enzyme E2Kb (UBC1 homolog, yeast) Protein, ubiquitin-conjugating enzyme E2Ka (UBC1 homolog, yeast) Protein, UBE2K Protein, Ube2k Protein, ube2k.S Protein, ube2k Protein, ube2kb Protein, ube2ka Protein
- Background
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Recombinant Human Ubiquitin-Conjugating Enzyme E2 K/UBE2K is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Asn200) of Human UBE2K fused with a 6His tag at the N-terminus.
Ubiquitin-Conjugating Enzyme E2 K (UBE2K) belongs to the E2 Ubiquitin-Conjugating Enzyme family. UBE2K is highly expressed in the brain, with highest levels found in cortex and striatum, and at lower levels in cerebellum and brainstem. UBE2K may mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. UBE2K is associated with the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. In addition, UBE2K is involved in Alzheimer's disease, Huntington's disease and antigen processing through its interaction with huntingtin, and MHC-heavy chain proteins. - Molecular Weight
- 34.5 kDa
- UniProt
- P61086
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