UBE2I Protein (AA 1-158) (GST tag)
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- Target See all UBE2I Proteins
- UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-158
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This UBE2I protein is labelled with GST tag.
- Purpose
- Recombinant Human Ubiquitin-Conjugating Enzyme E2 I/UBE2I (N-GST)
- Sequence
- MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSHMSG IALSRLAQER KAWRKDHPFG FVAVPTKNPD GTMNLMNWEC AIPGKKGTPW EGGLFKLRML FKDDYPSSPP KCKFEPPLFH PNVYPSGTVC LSILEEDKDW RPAITIKQIL LGIQELLNEP NIQDPAQAEA YTIYCQNRVE YEKRVRAQAK KFAPS
- Characteristics
- Recombinant Human Ubiquitin-Conjugating Enzyme E2 I/UBE2I is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Ser158) of Human UBE2I fused with a GST tag at the N-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product UBE2I Protein
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, pH 7.5.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))
- Alternative Name
- UBE2I (UBE2I Products)
- Synonyms
- C358B7.1 Protein, P18 Protein, UBC9 Protein, ubc9 Protein, ube2ia Protein, zUbc9 Protein, 5830467E05Rik Protein, F830028O17Rik Protein, Ubce2i Protein, Ubce9 Protein, UbcE2A Protein, ubce9 Protein, T13J8.70 Protein, T13J8_70 Protein, UBIQUITIN-PROTEIN LIGASE Protein, ubiquitin conjugating enzyme 9 Protein, ubiquitin conjugating enzyme E2 I Protein, ubiquitin-conjugating enzyme E2Ib Protein, ubiquitin-conjugating enzyme E2L Protein, ubiquitin-conjugating enzyme E2I Protein, ubiquitin conjugating enzyme E2I S homeolog Protein, ubiquitin conjugating enzyme 9 Protein, SUMO-conjugating enzyme UBC9 Protein, UBE2I Protein, ube2ib Protein, UBE2L Protein, Ube2i Protein, ube2i.S Protein, UBC9 Protein, ubc-9 Protein, LOC108703134 Protein
- Sub Type
- Fusionprotein
- Background
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SUMO-Conjugating Enzyme UBC9 (UBC9) belongs to the ubiquitin-conjugating enzyme family. UBC9 is homologous to ubiquitin-conjugating enzymes (E2s). However, instead of conjugating ubiquitin, UBC9 conjugates a ubiquitin homologue, Small Ubiquitin-Like Modifier 1 (SUMO-1). The conjugation of ubiquitin requires the activities of ubiquitin-activating (E1) and conjugating (E2) enzymes. It is suggested that UBC9 might play a role in DNA repair and perhaps even in aging.
Alternative Names: SUMO-Conjugating Enzyme UBC9, SUMO-Protein Ligase, Ubiquitin Carrier Protein 9, Ubiquitin Carrier Protein I, Ubiquitin-Conjugating Enzyme E2 I, Ubiquitin-Protein Ligase I, p18, UBE2I, UBC9, UBCE9 - Molecular Weight
- 44.4 kDa
- UniProt
- P63279
- Pathways
- Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Ubiquitin Proteasome Pathway
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