CTCF Protein (AA 1-154)

Catalog No. ABIN1691993

Product Details

Target: CTCF (CCCTC-Binding Factor (Zinc Finger Protein) (CTCF))

Protein Type: Recombinant

Protein Characteristics: AA 1-154

Origin: Human

Source: Escherichia coli (E. coli)

Purpose: Recombinant Human Transcriptional Repressor CTCF/CTCF

Sequence: MEGDAVEAIV EESETFIKGK ERKTYQRRRE GGQEEDACHL PQNQTDGGEV VQDVNSSVQM VMMEQLDPTL LQMKTEVMEG TVAPEAEAAV DDTQIITLQV VNMEEQPINI GELQLVQVPV PVTVPVATTS VEELQGAYEN EVSKEGLAES EPMI

Characteristics:

Recombinant Human Transcriptional Repressor CTCF/CTCF

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CTCF (CCCTC-Binding Factor (Zinc Finger Protein) (CTCF))

Alternative Name: Transcriptional Repressor CTCF/CTCF (CTCF Products)

Synonyms: im:7152666 Protein, CTCF Protein, xctcf Protein, AW108038 Protein, CCCTC-binding factor Protein, CCCTC-binding factor (zinc finger protein) Protein, CTCF Protein, ctcf Protein, Ctcf Protein

Background: Recombinant Human Transcriptional Repressor CTCF/CTCF is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Ile154) of Human CTCF.
Transcriptional Repressor CTCF (CTCF) belongs to the CTCF Zinc-Finger Protein family. CTCF contains twelve C2H2-type zinc fingers and interacts with CHD8. CTCF is widely expressed in many tissues, and it is absent in primary spermatocytes. CTCF is involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. CTCF plays an essential role in oocyte and preimplantation embryo development by activating or repressing transcription. In addition, CTCF is also indispensable in the epigenetic regulation and chromatin remodeling.

Molecular Weight: 16.9 kDa

UniProt: P49711