IL-13 Protein (AA 22-131) (His tag)

Catalog No. ABIN1691699

Product Details

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Protein Type: Recombinant

Protein Characteristics: AA 22-131

Origin: Mouse

Source: Human Cells

Purification tag / Conjugate: This IL-13 protein is labelled with His tag.

Purpose: Recombinant Mouse Interleukin-13/IL-13 (Pro22-Phe131,C-6His)

Sequence: PVPRSVSLPL TLKELIEELS NITQDQTPLC NGSMVWSVDL AAGGFCVALD SLTNISNCNA IYRTQRILHG LCNRKAPTTV SSLPDTKIEV AHFITKLLSY TKQLFRHGPF VDHHHHHH

Characteristics:

Recombinant Mouse Interleukin-13/IL-13 (Pro22-Phe131,C-6His)

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Alternative Name: Interleukin-13/IL-13 (IL13 Products)

Background: Recombinant Mouse Interleukin-13/IL-13 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Pro22-Phe131) of Mouse IL-13 fused with a polyhistidine tag at the C-terminus.
Mouse interleukin 13 (mIL-13) is a pleiotropic cytokine produced by activated Th2 cells. IL-13 induces B cell proliferation and immunoglobin production. It contains a four helical bundle with two internal disulfide bonds. Mouse IL13 shares 58 % sequence identity with human protein and exhibits cross-species activity. IL13 signals via receptor IL13R (type2, IL4R) and activates STAT-6. IL13 initially binds IL-13Rα1 with low affinity and triggers association of IL4Rα, generating a high affinity heterodimeric receptor IL13R and eliciting downstream signals. IL13 also binds IL-13Rα2 with high affinity, which plays a role in a negative feedback system of IL13 signaling. IL13 is an important mediator of allergic inflammation and disease.

Molecular Weight: 13.1 kDa

UniProt: P20109

Pathways: JAK-STAT Signaling, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport