IL-7 Protein (AA 26-177) (His tag)
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- Target See all IL-7 (IL7) Proteins
- IL-7 (IL7) (Interleukin 7 (IL7))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 26-177
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Origin
- Human
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Source
- Human Cells
- Purification tag / Conjugate
- This IL-7 protein is labelled with His tag.
- Purpose
- Recombinant Human Interleukin-7/IL-7 (C-6His)
- Sequence
- DCDIEGKDGK QYESVLMVSI DQLLDSMKEI GSNCLNNEFN FFKRHICDAN KEGMFLFRAA RKLRQFLKMN STGDFDLHLL KVSEGTTILL NCTGQVKGRK PAALGEAQPT KSLEENKSLK EQKKLNDLCF LKRLLQEIKT CWNKILMGTK EHVDHHHHHH
- Characteristics
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Recombinant Human Interleukin-7/IL-7 (C-6His)
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product IL7 Protein
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Interleukin 7 (IL7) (AA 26-177) protein
IL7 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Interleukin 7 (IL7) (AA 26-154) (Active) protein (His tag)IL7 Origin: Mouse Host: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE. Active
Interleukin 7 (IL7) (AA 26-177) (Active) protein (His tag)IL7 Origin: Human Host: Escherichia coli (E. coli) > 95 % AcA, CC Active
Interleukin 7 (IL7) (AA 26-154) protein (His tag)IL7 Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 7 (IL7) (AA 26-177) protein (His-IF2DI Tag)IL7 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 7 (IL7) (AA 26-177) protein (GST tag)IL7 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Interleukin 7 (IL7) (Active) protein (His tag)IL7 Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE Active
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- 4 °C/-20 °C/-80 °C
- Storage Comment
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- IL-7 (IL7) (Interleukin 7 (IL7))
- Alternative Name
- Interleukin-7/IL-7 (IL7 Products)
- Background
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Recombinant Human Interleukin-7/IL-7 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Asp26-His177) of Human IL-7 fused with a polyhistidine tag at the C-terminus.
Human Interleukin 7 (IL-7) is a potent lymphoid cell growth factor stimulating the proliferation of lymphoid progenitors. IL7 can associate with the hepatocyte growth factor (HGF) to form a hybrid cytokine that functions as a pre-pro-B cell growth-stimulating factor. Human IL7 cDNA encodes a 177 AA precursor protein containing a 25 AA signal peptide and a 152 AA mature protein. Human and mouse IL7 share 65 % sequence identity in the mature region and both exhibit cross-species activity. IL-7 signals via IL-7 receptor (IL7R) activating multiple pathways including JaK/STAT and PI3K/AKT, which regulate lymphocyte survival, glucose uptake, proliferation, and differentiation. IL-7 is also associated with cytoplasmic IL2-R γ for signal transduction. - Molecular Weight
- 18.4 kDa
- UniProt
- P13232
- Pathways
- JAK-STAT Signaling
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