Protein phosphatase 1 (AA 1-171), (Regulatory Subunit 1A) protein (His tag)

Catalog No. ABIN1690904

Product Details

Target: Protein phosphatase 1

Protein Type: Recombinant

Protein Characteristics: AA 1-171, Regulatory Subunit 1A

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: His tag

Purpose: Recombinant Human Protein Phosphatase 1 Regulatory Subunit 1A/PPP1R1A (C-6His)

Sequence: MEQDNSPQKI QFTVPLLEPH LDPEAAEQIR RRRPTPATLV LTSDQSSPEI DEDRIPNPHL KSTLAMSPRQ RKKMTRITPT MKELQMMVEH HLGQQQQGEE PEGAAESTGT QESRPPGIPD TEVESRLGTS GTAKKTAECI PKTHERGSKE PSTKEPSTHI PPLDSKGANS VLEHHHHHH

Characteristics: Recombinant Human Protein Phosphatase 1 Regulatory Subunit 1A/PPP1R1A is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Val171) of Human PPP1R1A.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl, 1 mM DTT, 50 % Glycerol, pH 8.5.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: Protein phosphatase 1

Background: Protein Phosphatase 1 Regulatory Subunit 1A (PPP1R1A) is an inhibitor of protein-phosphatase 1. PPP1R1A is a cellular regulator of eIF2 alpha phosphorylation. In hormonal control of glycogen metabolism, IPP-1 protein plays important function. Hormones can elevate intracellular cAMP level and elevate IPP-1 activity. PPP1R1A activation caused cAMP increase , cAMP control over proteins that are not directly phosphorylated by PKA following a rise in intracellular calcium. IPP-1 is inactivated by calcineurin (PP2B). Multiple domains in IPP-1 target cellular PP1 complexes.
Alternative Names: Protein Phosphatase 1 Regulatory Subunit 1A, Protein Phosphatase Inhibitor 1, I-1, IPP-1, PPP1R1A, IPP1

Molecular Weight: 19.98 kDa

UniProt: Q13522