CA5B Protein (AA 34-317) (His tag)
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- Target See all CA5B Proteins
- CA5B (Carbonic Anhydrase VB, Mitochondrial (CA5B))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 34-317
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This CA5B protein is labelled with His tag.
- Purpose
- Recombinant Human Carbonic Anhydrase 5B/CA5B (C-6His)
- Sequence
- MCSLYTCTYK TRNRALHPLW ESVDLVPGGD RQSPINIRWR DSVYDPGLKP LTISYDPATC LHVWNNGYSF LVEFEDSTDK SVIKGGPLEH NYRLKQFHFH WGAIDAWGSE HTVDSKCFPA ELHLVHWNAV RFENFEDAAL EENGLAVIGV FLKLGKHHKE LQKLVDTLPS IKHKDALVEF GSFDPSCLMP TCPDYWTYSG SLTTPPLSES VTWIIKKQPV EVDHDQLEQF RTLLFTSEGE KEKRMVDNFR PLQPLMNRTV RSSFRHDYVL NVQAKPKPAT SQATPLEHHH HHH
- Characteristics
- Recombinant Human Carbonic Anhydrase 5B/CA5B is produced with our E. coli expression system. The target protein is expressed with sequence (Cys34-Pro317) of Human CA5B.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product CA5B Protein
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Carbonic Anhydrase VB, Mitochondrial (CA5B) (AA 1-317) protein (GST tag)
CA5B Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Carbonic Anhydrase VB, Mitochondrial (CA5B) (AA 2-90) protein (GST tag)CA5B Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Carbonic Anhydrase VB, Mitochondrial (CA5B) proteinCA5B Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Carbonic Anhydrase VB, Mitochondrial (CA5B) protein (Myc-DYKDDDDK Tag)CA5B Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 100 mM NaCl, pH 8.0.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- CA5B (Carbonic Anhydrase VB, Mitochondrial (CA5B))
- Alternative Name
- ca5b (CA5B Products)
- Synonyms
- CA-VB Protein, 7330410H16Rik Protein, CAVB Protein, Ca5b Protein, CarVb Protein, D730005F19Rik Protein, zgc:171653 Protein, CA5B Protein, ca5b Protein, carbonic anhydrase 5B Protein, carbonic anhydrase 5b, mitochondrial Protein, carbonic anhydrase Va Protein, carbonic anhydrase 5A Protein, carbonic anhydrase 5B, mitochondrial Protein, carbonic anhydrase VB, mitochondrial L homeolog Protein, CA5B Protein, Car5b Protein, Ca5b Protein, ca5a Protein, CA5A Protein, LOC100051095 Protein, ca5b.L Protein
- Background
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Carbonic Anhydrase 5B (CA5B) is a member of alpha-carbonic anhydrase family (CAs) that catalyze the reversible hydration of carbon dioxide. CAs is associated with many biological processes, including calcification, respiration, bone resorption, acid-base balance and the formation of aqueous humor. CA5B is highly expressed in heart, pancreas, kidney, placenta, lung, and skeletal muscle, but it is restricted to the liver. CA5B is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA, CA-VA. CA5B is inhibited by coumarins, sulfonamide derivatives such as acetazolamide (AZA), saccharin, and Foscarnet.
Alternative Names: Carbonic Anhydrase 5B Mitochondrial, Carbonate Dehydratase VB, Carbonic Anhydrase VB, CA-VB, CA5B - Molecular Weight
- 33.77 kDa
- UniProt
- Q9Y2D0
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