AHSA1 Protein

Catalog No. ABIN1686715

Product Details

Target: AHSA1 (Activator of HSP90 ATPase Activity 1 (AHSA1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), SDS-PAGE (SDS), Functional Studies (Func)

Sequence: SHMAKWGEGD PRWIVEERAD ATNVNNWHWT ERDASNWSTD KLKTLFLAVQ VQNEEGKCEV TEVSKLDGEA SINNRKGKLI FFYEWSVKLN WTGTSKSGVQ YKGHVEIPNL SDENSVDEVE ISVSLAKDEP DTNLVALMKE EGVKLLREAM GIYISTLKTE FTQGMILPTM NGESVDPVGQ PALKTEERKA KPAPSKTQAR PVGVKIPTCK ITLKETFLTS PEELYRVFTT QELVQAFTHA PATLEADRGG KFHMVDGNVS GEFTDLVPEK HIVMKWRFKS WPEGHFATIT LTFIDKNGET ELCMEGRGIP APEEERTRQG WQRYYFEGIK QTFGYGARLF

Specificity: ~38 kDa

Characteristics: 2 µM ABIN1686714 generated a 9-fold ATPase activation of 2 µM Hsp90 (His-tagged Hsp90 beta) in 33 mM Hepes pH 7.2, 30 mM NaCl, 5 mM MgCl2, 1 mM DTT, 1.5 mM ATP in a 100 µL reaction at 37 degrees C. (This is an enzyme-linked ATP regeneration assay tracking loss of NADH absorbance at 340nm.)

Purification: Multi-Step Purified

Purity: >90%

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product has been certified >90% pure using SDS PAGE analysis. 2uM ABIN1686714 generated a 9-fold ATPase activation of 2uM HSP90 (His-tagged HSP90 beta) in 33mM Hepes pH7.2, 30mM NaCl, 5mM MgCl2, 1mM DTT, 1.5mM ATP in a 100ul reaction at 37 degrees C. (This is an enzyme-linked ATP regeneration assay tracking loss of NADH absorbance at 340nm.)

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: 20 mM HEPES buffer pH 7.2, 80 mM NaCl, 10 % glycerol

Storage: -20 °C

References

Margarucci, Monti, Cassiano, Mozzicafreddo, Angeletti, Riccio, Tosco, Casapullo: "Chemical proteomics-driven discovery of oleocanthal as an Hsp90 inhibitor." in: Chemical communications (Cambridge, England), Vol. 49, Issue 52, pp. 5844-6, (2013) (PubMed).

Target Details

Target: AHSA1 (Activator of HSP90 ATPase Activity 1 (AHSA1))

Alternative Name: Aha1 (AHSA1 Products)

Synonyms: ahsa1 Protein, MGC52884 Protein, MGC132147 Protein, MGC80312 Protein, AHSA1 Protein, MGC89589 Protein, wu:fb83b05 Protein, zgc:136821 Protein, zgc:56075 Protein, AHA1 Protein, C14orf3 Protein, p38 Protein, BC023857 Protein, AHA1, activator of heat shock 90kDa protein ATPase homolog 1 S homeolog Protein, activator of Hsp90 ATPase activity 1 Protein, AHA1, activator of heat shock 90kDa protein ATPase homolog 1 L homeolog Protein, activator of HSP90 ATPase activity 1 Protein, AHA1, activator of heat shock 90kDa protein ATPase homolog 1 Protein, AHA1, activator of heat shock protein ATPase homolog 1a Protein, AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast) Protein, AHA1, activator of heat shock protein ATPase 1 Protein, ahsa1.S Protein, AHSA1 Protein, ahsa1.L Protein, ahsa1 Protein, ahsa1a Protein, Ahsa1 Protein

Background: Aha1 is a member of the HSP90 cochaperone family, and is thought to stimulate HSP90 ATPase activity by competing with p23 and other co-chaperones for HSP90 binding (1, 2). It may affect a step in the endoplasmic reticulum to Golgi trafficking. Aha1 also interacts with HSPCA/HSP90 and with the cytoplasmic tail of the vesicular stomatistis virus glycoproteins (VSV G) (3). Aha1 is expressed in numerous tissues, including the brain, heart, skeletal muscle, and kidney, and at low levels, the liver and placenta. Aha1 might be a potential therapeutic strategy to increase sensitivity to HSP inhibitors (4).

Molecular Weight: approx. 38 kDa

Gene ID: 10598

NCBI Accession: NP_036243

UniProt: O95433