IL18R1 Protein (C-Term, Extracellular Domain)

Catalog No. ABIN1684708

Product Details

Target: IL18R1 (Interleukin 18 Receptor 1 (IL18R1))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: C-Term, Extracellular Domain

Origin: Mouse

Source: HEK-293T Cells

Specificity: Optimized DNA sequence encoding extracellular domain of Mouse IL-18 receptor including an C terminal IgG1 Fc tag was expressed in HEK293 cells.

Characteristics: Recombinant mouse IL-18 receptor alpha is a homodimer protein consisting of two 555 amino acid residue subunits, due to glycosylation migrates as an approximately 95kD protein under reducing SDS-PAGE conditions.

Purity: > 98 %, as determined by SDS-PAGE and HPLC

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

Application Details

Comment:

Biologically active: Measured by its ability to bind immobilized recombinant human IL-18 at a concentration range of 1-32 ng/mL in a functional ELISA assay.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS solution, pH7.2

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: The lyophilized protein is stable for at least years from date of receipt at -20 °C. Upon reconstitution, this cytokine can be stored in working aliquots at - 8 °C for one month, or at -20 °C for six months, with a carrier protein without detectable loss of activity.

Expiry Date: 12-24 months

References

Ebel, Awe, Kaplan, Kansas: "Diverse inflammatory cytokines induce selectin ligand expression on murine CD4 T cells via p38α MAPK." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 194, Issue 12, pp. 5781-8, (2015) (PubMed).

Target Details

Target: IL18R1 (Interleukin 18 Receptor 1 (IL18R1))

Alternative Name: IL18R1 (IL18R1 Products)

Synonyms: CD218a Protein, CDw218a Protein, IL-1Rrp Protein, IL18RA Protein, IL1RRP Protein, IL-1R9 Protein, IL1R9 Protein, IL1RAPL-2 Protein, TIGIRR-1 Protein, Il18ralpha Protein, Il1rrp Protein, IL-18Ra Protein, IL18R1 Protein, interleukin 18 receptor 1 Protein, interleukin 1 receptor accessory protein like 2 Protein, IL18R1 Protein, IL1RAPL2 Protein, Il18r1 Protein

Background: PDGF is synthesized mainly by megakaryocytes. It is stored in the alpha granules of platelets from which it is released after cell activation of platelets. Platelets synthesize a mixture of the three possible isoforms (BB,AB,AA) while fibroblasts stimulated with EGF synthesize AA homodimers. PDGF receptors are expressed in fibroblasts, osteoblasts, chondroblasts, smooth muscle cells, glial cells, and endothelial cells. Two related receptors, are PDGFRalpha (CD140a) or PDGFRbeta ( CD140b). In contrast to many other cytokines PDGF is not released into the circulation. PDGF binds to several plasma proteins and also to proteins of the extracellular matrix which facilitates local concentration of the factor. The factor functions as a local autocrine and paracrine growth factor. In the adult organism PDGF is involved in wound healing processes. The aberrant expression of PDGF is observed with vascular proliferative diseases such as atherosclerosis. PDGF regulates the synthesis of its own receptor and also influences the expression of membrane receptors for IL1, EGF, 5-Hydroxytryptamine, LDL, transferrin, and muscarinergic receptor.

UniProt: Q61098