IL1R1 Protein (C-Term, Extracellular Domain)

Catalog No. ABIN1684651

Product Details

Target: IL1R1 (Interleukin 1 Receptor, Type I (IL1R1))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: C-Term, Extracellular Domain

Origin: Mouse

Source: HEK-293T Cells

Specificity: Optimized DNA sequence encoding Human extracellular domain of mouse IL-1 receptor type 1 including a C-terminal His tag was expressed in HEK293 cells.

Characteristics: Recombinant IL-R1 (CD121a) is a protein consisting of 330 amino acid residue subunits, due to glycosylation migrates as an approximately 55-60 kDa protein on SDS-PAGE.

Purity: > 97 %, as determined by SDS-PAGE and HPLC

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

Application Details

Comment:

Recombinant mouse IL1R1 activity was measured by its ability to bind mouse IL1B in a functional ELISA. Immobilized mouse IL1A at 10 µg/mL (100 µL/well) can bind mouse IL1R1, The EC50 of mouse IL1R1 is 0.13 µg/mL.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS solution, pH 7.2, 5 % Trehalose.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: The lyophilized protein is stable for at least years from date of receipt at -20 °C. Upon reconstitution, this cytokine can be stored in working aliquots at - 8 °C for one month, or at -20 °C for six months, with a carrier protein without detectable loss of activity.

Expiry Date: 12-24 months

Target Details

Target: IL1R1 (Interleukin 1 Receptor, Type I (IL1R1))

Alternative Name: CD121a (IL1R1 Products)

Synonyms: CD121A Protein, D2S1473 Protein, IL-1R-alpha Protein, IL1R Protein, IL1RA Protein, P80 Protein, CD121a Protein, CD121b Protein, IL-iR Protein, Il1r-1 Protein, DER4 Protein, Fit-1 Protein, Ly84 Protein, ST2L Protein, St2 Protein, St2-rs1 Protein, T1 Protein, T1/ST2 Protein, interleukin 1 receptor type 1 Protein, interleukin 1 receptor, type I Protein, interleukin 1 receptor-like 1 Protein, IL1R1 Protein, Il1r1 Protein, Il1rl1 Protein

Background: Eotaxin belongs to the platelet factor-4 family of chemokines. The gene symbol is SCYA11. The factor is being referred to also as Eotaxin-1 and has been renamed CCL11. Eotaxin induces substantial accumulation of eosinophils in the skin without significantly affecting the accumulation of neutrophils. Eotaxin is a potent stimulator of both guinea pig and human eosinophils in vitro. The Eotaxin receptor is a G-protein-coupled receptor selectively expressed in human eosinophils.

UniProt: P13504

Pathways: NF-kappaB Signaling, Carbohydrate Homeostasis, Cancer Immune Checkpoints