gamma Globulin Fraction Protein
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- Target
- gamma Globulin Fraction
- Protein Type
- Native
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Origin
- Mouse
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Source
- Mouse
- Application
- Control (Ct), SDS-PAGE (SDS), ELISA
- Purpose
- Mouse Gamma Globulin Fraction
- Purification
- Mouse Gamma Globulin was prepared from normal Mouse serum by a multi-step process which includes delipidation and salt fractionation followed by extensive dialysis against the buffer stated above.
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- Application Notes
- Mouse gamma globulin blocking reagent has been tested by SDS-PAGE and is an ideal blocker for western blotting, ELISA, Immunohistochemistry and other detection assays.
- Comment
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Assay by immunoelectrophoresis resulted in precipitin arcs against anti-Mouse Serum corresponding to gamma globulins.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Restore with deionized water (or equivalent)
- Concentration
- 20.0 mg/mL
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid cycles of freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store gamma globulin at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- gamma Globulin Fraction
- Background
- Gamma globulins are a class of globulins, identified by their position after serum protein electrophoresis. The most significant gamma globulins are immunoglobulins ("Igs"), more commonly known as antibodies, although some Igs are not gamma globulins, and some gamma globulins are not Igs. Globulin is one of the three main types of serum proteins, the others being albumin and fibrinogen. Some globulins are produced in the liver, while others are made by the immune system.
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