HCP Protein
HCP
Origin: E. coli
Host: Escherichia coli (E. coli)
Native
SDS, 2D-PAGE, WB
2 images
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- Target See all HCP products
- HCP (Host Cell Proteins (HCP))
- Protein Type
- Native
- Origin
- E. coli
- Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS), Two Dimensional Polyacrylamide Gel Electrophoresis (2D-PAGE), Western Blotting (WB)
- Purpose
- E.coli Host Cell Protein Control Protein (HCP) (in 1x Loading Buffer)
- Specificity
- E. coli Host Cell Protein Control Protein was prepared by solubilizing mock induced E. coli (BL21) cells.
- Characteristics
- Host Cell Protein Lysate was prepared by solubilizing mock induced E.coli cells. Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate. Synonyms: E.coli Host Cell Proteins , HCP antigen, E.coli control antigen
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- Application Notes
- Western Blot Dilution: 1:500 - 1:1000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 2.0 mg/mL
- Buffer
- 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2 % SDS, 10 % Glycerol and 0.005 % bromophenol blue, pH 6.8)
- Preservative
- Without preservative
- Storage
- -20 °C
- Storage Comment
- Store Host Cell Proteins control protein at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- HCP (Host Cell Proteins (HCP))
- Alternative Name
- Host Cell Proteins (HCP Products)
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