TIE1 Protein (glycosylated, Homodimer, Soluble) (Fc Tag)

Catalog No. ABIN1589960

Product Details

Target: TIE1 (tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1))

Protein Type: Recombinant

Protein Characteristics: glycosylated, Homodimer, Soluble

Origin: Mouse

Source: CHO Cells

Purification tag / Conjugate: This TIE1 protein is labelled with Fc Tag.

Sequence: VDLTLLANLR ITDPQRFFLT CVSGEAGAGR SSDPPLLLEK DDRIVRTFPP GQPLYLARNG SHQVTLRGFS KPSDLVGVFS CVGGAGARRT RVLYVHNSPG AHLFPDKVTH TVNKGDTAVL SAHVHKEKQT DVIWKNNGSY FNTLDWQEAD DGRFQLQLQN VQPPSSGIYS ATYLEASPLG SAFFRLIVRG CGAGRWGPGC VKDCPGCLHG GVCHDHDGEC VCPPGFTGTR CEQACREGRF GQSCQEQCPG TAGCRGLTFC LPDPYGCSCG SGWRGSQCQE ACAPDHFGAD CRLQCQCQNG GTCDRFSGCV CPSGWHGVHC EKSDRIPQIL SMATEVEFNI GTMPRINCAA AGNPFPVRGS MKLRKPDGTM LLSTKVIVEP DRTTAEFEVP SLTLGDSGFW ECRVSTSGGQ DSRRFKVNVK VPPVPLTAPR LLAKQSRQLV VSPLVSFSGD GPISSVRLHY RPQDSTIAWS AIVVDPSENV TLMNLKPKTG YNVRVQLSRP GEGGEGGWGP SALMTTDCPE PLLQPWLESW HVEGPDRLRV SWSLPSVPLS GDGFLLRLWD GARGQERREN ISFPQARTAL LTGLTPGTHY QLDVRLYHCT LLGPASPPAH VHLPPSGPPA PRHLHAQALS DSEIQLMWQH PEAPSGPISK YIVEIQVAGG SGDPQWMDVD RPEETSIIVR GLNASTRYLF RVRASVQGLG DWSNTVEEAT LGNGLQSEDP VRESRAAEEG LTRSDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRE EMTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP MLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG K

Characteristics: Length (AA): 957
Chromosomal location: 4 D2.1, 4 50.0 cM

Purity: > 90 % by SDS-PAGE. Visualized by silver stain

Application Details

Comment:

Soluble Receptors

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: The lyophilized sTIE-1/Fc is soluble in water and most aqueous buffers and should be reconstituted in PBS or medium to a concentration not lower than 50 μg/mL.

Buffer: PBS

Storage: -20 °C/-80 °C

Storage Comment: Lyophilized samples are stable for greater than six months at -20 °C to -70 °C. Reconstituted sTIE-1/Fc should be stored in working aliquots at -20 °C.

Expiry Date: 6 months

Target Details

Target: TIE1 (tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1))

Alternative Name: TIE-1 (TIE1 Products)

Synonyms: JTK14 Protein, TIE Protein, D430008P04Rik Protein, tie-1 Protein, tyrosine kinase with immunoglobulin like and EGF like domains 1 Protein, tyrosine kinase with immunoglobulin-like and EGF-like domains 1 Protein, tyrosine-protein kinase receptor Tie-1 Protein, endothelium-specific receptor tyrosine kinase 1 Protein, TIE1 Protein, Tie1 Protein, CpipJ_CPIJ018933 Protein, tie1 Protein

Background: Recombinant murine soluble TIE-1 was fused with the Fc part of human IgG1. The recombinant mature sTIE-1/hFc is a disulfide-linked homodimeric protein. The sTIE-1/hFc monomers have a mass of approximately 105 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 130 kDa protein in SDS-PAGE under reducing conditions. The soluble receptor protein consists of the full extracellular domain (Val23-Glu749). TIE-1 (tyrosine kinase with Ig and EGF homology domains 1) and TIE-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis. Murine TIE-1 cDNA encodes a 1134 amino acid (aa) residue precursor protein with an 22 residue putative signal peptide, a 733 residue extracellular domain and a 354 residue cytoplasmic domain. Whereas two ligands have been described for TIE-2 [angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2)], so far no ligand was found for TIE-1.
Synonyms: Tie1, TIE, tie-1, D430008P04Rik

Molecular Weight: 260.0k Da

NCBI Accession: NP_035717, NM_011587

UniProt: Q06806

Pathways: RTK Signaling