VEGFC Protein (His tag)
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- Target See all VEGFC Proteins
- VEGFC (Vascular Endothelial Growth Factor C (VEGFC))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- Insect Cells
- Purification tag / Conjugate
- This VEGFC protein is labelled with His tag.
- Purpose
- VEGF-C
- Sequence
- DPTEETIKFA AAHYNTEILK SIDNEWRKTQ CMPREVCIDV GKEFGVATNT FFKPPCVSVY RCGGCCNSEG LQCMNTSTSY LSKTLFEITV PLSQGPKPVT ISFANHTSCR CMSKLHHHHH H
- Specificity
- Chromosomal location:11q13
- Characteristics
- Length (aa):121
- Purity
- > 90 % by SDS-PAGE
- Top Product
- Discover our top product VEGFC Protein
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- Application Notes
- The biological activity was determined (i) by the ability to induce VEGFR-3/FLT-4 receptor phosphorylation in PAEC/VEGFR-3 cells and (ii) the VEGF-C-induced proliferation of primary human dermal lymphatic endothelial cells (HDLEC).
- Comment
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Cytokines & Growth Factors
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- The lyophilized VEGF-C is soluble in water and most aqueous buffers. The lyophilized VEGF-C should be reconstituted in PBS or medium to a concentration not lower than 50 μg/mL.
- Buffer
- Water
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -20 °C,-80 °C
- Storage Comment
- Lyophilized samples are stable for more than six months at -20°C to -70°C. Reconstituted VEGF-C should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 6 months
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- Target
- VEGFC (Vascular Endothelial Growth Factor C (VEGFC))
- Alternative Name
- VEGF-C (VEGFC Products)
- Synonyms
- VEGFC Protein, vegf-c Protein, xvegf-c Protein, Flt4-L Protein, VRP Protein, AW228853 Protein, VEGF-C Protein, vascular endothelial growth factor c Protein, vascular endothelial growth factor C Protein, vascular endothelial growth factor C S homeolog Protein, vegfc Protein, VEGFC Protein, vegfc.S Protein, Vegfc Protein
- Background
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VEGF-C, also known as Vascular Endothelial Growth Factor Related Protein (VRP), is a recently discovered VEGF growth factor family member that is most closely related to VEGF-D. The human VEGF-C cDNA encodes a pre-pro-protein of 416 amino acids residues. It is almost identical to the mouse VEGF-C protein. Similar to VEGF-D, VEGF-C has a VEGF homology domain spanning the middle third of the precursor molecule and long N- and C-terminal extensions. In adults, VEGF-C is highly expressed in heart, placenta, ovary and small intestine. Recombinant human VEGF-C, lacking the N- and C-terminal extensions and containing only the middle VEGF homology domain, forms primarily non-covalently linked dimers. This protein is a ligand for both VEGFR-2/KDR and VEGFR-3/FLT-4. Since VEGFR-3 is strongly expressed in lymphatic endothelial cells, it has been postulated that VEGF-C is involved in the regulation of the growth and/or differentiation of lymphatic endothelium. Although recombinant human VEGF-C is also a mitogen for vascular endothelial cells, it is much less potent than VEGF-A. The recombinant human VEGF-C contains 121 amino acids residues and was fused to a His-tag (6x His) at the C-terminal end. As a result of glycosylation VEGF-C migrates as an 18-24 kDa protein in SDS-PAGE under reducing conditions.
Synonyms: vascular endothelial growth factor C, VEGFC, VRP, Flt4-L - Molecular Weight
- 18.0-24.0 kDa
- Gene ID
- 7424
- NCBI Accession
- NM_005429, NP_005420
- UniProt
- P49767
- Pathways
- RTK Signaling, Signaling Events mediated by VEGFR1 and VEGFR2
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