Podoplanin Protein (PDPN) (Soluble) (His tag)

Catalog No. ABIN1589761

Product Details

Target: Podoplanin (PDPN)

Protein Type: Recombinant

Protein Characteristics: Soluble

Origin: Rat

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This Podoplanin protein is labelled with His tag.

Purpose: Podoplanin, soluble

Sequence: GAIGALEDDL VTPGPGDDMV NPGLEDRIET TDTTGELDKS TAKAPLVPTQ PPIEELPTSG TSDHDHKEHE STTTVKAVTS HSTDKKTTHP NRDNAGGETQ TTDKKDGLAV VTLEHHHHHH

Specificity: Chromosomal location:5q36

Characteristics: Length (aa):120

Purity: > 98 % by SDS-PAGE

Application Details

Comment:

Soluble Receptors

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: We recommend a quick spin followed by reconstitution in water to a concentration of 0.1-1.0 mg/mL. This solution can then be diluted into other aqueous buffers and stored at 4 °C for 1 week or -20 °C for future use.

Buffer: 0.5X PBS

Handling Advice: Centrifuge vial prior to opening.

Storage: RT,-20 °C

Storage Comment: The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C. Reconstituted sPodoplanin should be stored in working aliquots at -20°C.

Target Details

Target: Podoplanin (PDPN)

Alternative Name: Podoplanin (PDPN Products)

Synonyms: PDPN Protein, AGGRUS Protein, GP36 Protein, GP40 Protein, Gp38 Protein, HT1A-1 Protein, OTS8 Protein, PA2.26 Protein, T1A Protein, T1A-2 Protein, OTS-8 Protein, RANDAM-2 Protein, T1-alpha Protein, T1a Protein, T1alpha Protein, E11 Protein, RTI40 Protein, podoplanin Protein, PDPN Protein, Pdpn Protein

Background: Podoplanin, also known as glycoprotein 38 (gp38), PA2.26 antigen, T1alpha (T1A), and aggrus, is a 38 kDa type I transmembrane sialoglycoprotein and member of the podoplanin family. Podoplanin is synthesized as a 172 amino acid (aa) precursor with a 22 aa signal sequence, a 119 aa extracellular domain (ECD), a 21 aa transmembrane region, and a short, 10 aa cytoplasmic tail. The ECD contains abundant Ser/Thr residues as potential sites for Oglycosylation, and the cytoplasmic region contains putative sites for kinase C and cAMP phosphorylation. Mouse Podoplanin shares 77% and 46 % aa sequence identity with rat and human Podoplanin, respectively. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and on numerous tumors including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for Ctype lectin like receptor 2 (CLEC2). Their association is dependent on sialic acid on Oglycans of Podoplanin. Through its association with CLEC2, Podoplanin induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth.
Synonyms: Pdpn, E11, Gp38, OTS-8, RTI40, T1-alpha

Gene ID: 54320

NCBI Accession: NM_019358, NP_062231

UniProt: Q64294

Pathways: Dicarboxylic Acid Transport