TEK Protein (Dimer, glycosylated, Soluble) (Fc Tag)

Catalog No. ABIN1589758

Product Details

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Protein Type: Recombinant

Protein Characteristics: glycosylated, Dimer, Soluble

Origin: Mouse

Source: CHO Cells

Purification tag / Conjugate: This TEK protein is labelled with Fc Tag.

Purpose: TIE-2/Fc Chimera, soluble

Sequence: GAMDLILINS LPLVSDAETS LTCIASGWHP HEPITIGRDF EALMNQHQDP LEVTQDVTRE WAKKVVWKRE KASKINGAYF CEGRVRGQAI RIRTMKMRQQ ASFLPATLTM TVDRGDNVNI SFKKVLIKEE DAVIYKNGSF IHSVPRHEVP DILEVHLPHA QPQDAGVYSA RYIGGNLFTS AFTRLIVRRC EAQKWGPDCS RPCTTCKNNG VCHEDTGECI CPPGFMGRTC EKACEPHTFG RTCKERCSGP EGCKSYVFCL PDPYGCSCAT GWRGLQCNEA CPSGYYGPDC KLRCHCTNEE ICDRFQGCLC SQGWQGLQCE KEGRPRMTPQ IEDLPDHIEV NSGKFNPICK ASGWPLPTSE EMTLVKPDGT VLQPNDFNYT DRFSVAIFTV NRVLPPDSGV WVCSVNTVAG MVEKPFNISV KVLPEPLHAP NVIDTGHNFA IINISSEPYF GDGPIKSKKL FYKPVNQAWK YIEVTNEIFT LNYLEPRTDY ELCVQLARPG EGGEGHPGPV RRFTTASIGL PPPRGLSLLP KSQTALNLTW QPIFTNSEDE FYVEVERRSL QTTSDQQNIK VPGNLTSVLL SNLVPREQYT VRARVNTKAQ GEWSEELRAW TLSDILPPQP ENIKISNITD STAMVSWTIV DGYSISSIII RYKVQGKNED QHIDVKIKNA TVTQYQLKGL EPETTYHVDI FAENNIGSSN PAFSHELRTL PHSPASADLG TRSDKTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSREE MTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPM LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK

Specificity: Chromosomal location:4 C5, 4 43.34 cM

Characteristics: Length (aa):978

Purity: > 90 % by SDS-PAGE

Application Details

Comment:

Soluble Receptors

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge vial prior to opening. The lyophilized sTIE-2-His is soluble in water and most aqueous buffers and should be reconstituted in PBS or medium to a concentration not lower than 50 μg/mL.

Buffer: PBS

Storage: -20 °C,-80 °C

Storage Comment: Lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted sTIE-2/hFc should be stored in working aliquots at -20°C.

Expiry Date: 6 months

Target Details

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Alternative Name: TIE-2 (TEK Products)

Synonyms: TEK Protein, DKFZp469E064 Protein, AA517024 Protein, Cd202b Protein, Hyk Protein, Tie2 Protein, tie-2 Protein, CD202B Protein, TIE-2 Protein, TIE2 Protein, VMCM Protein, VMCM1 Protein, Tie-2 Protein, tie2 Protein, TEK receptor tyrosine kinase Protein, angiopoietin-1 receptor Protein, TEK tyrosine kinase, endothelial Protein, TEK Protein, LOC100408334 Protein, tek Protein, Tek Protein

Background: Recombinant murine soluble TIE-2 was fused with the Fc part of human IgG1. The recombinant mature sTIE-2/Fc is a disulfide-linked homodimeric protein. The sTIE-2/Fc monomers have a mass of approximately 105 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 140 kDa protein in SDS-PAGE under reducing conditions. The soluble receptor protein consists of the full extracellular domain (Val19-Leu740). TIE-1 (tyrosine kinase with Ig and EGF homology domains 1) and TIE-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis. Human TIE-1 cDNA encodes a 1122 amino acid (aa) residue precursor protein with an 18 residue putative signal peptide, a 726 residue extracellular domain and a 353 residue cytoplasmic domain. Two ligands, angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2), which bind TIE-2 with high-affinity have been identified. Ang2 has been reported to act as an antagonist for Ang1. Mice engineered to overexpress Ang2 or to lack Ang1 or Tie-1 display similar angiogenic defects.
Synonyms: Angiopoietin-1 receptor, Endothelial tyrosine kinase, HYK, STK1, Tunica interna endothelial cell kinase, Tyrosine kinase with Ig and EGF homology domains-2, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2, p140 TEK, CD202b, Tek, Hyk, Tie2, AA517024

Molecular Weight: 280.0 kDa

Gene ID: 21687

NCBI Accession: NM_013690, NP_038718

UniProt: Q02858

Pathways: RTK Signaling, Growth Factor Binding