TEK Protein (Soluble) (His tag)

Catalog No. ABIN1589754

Product Details

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Protein Type: Recombinant

Protein Characteristics: Soluble

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This TEK protein is labelled with His tag.

Purpose: TIE-2, soluble

Sequence: AMDLILINSL PLVSDAETLT CIASGWHPHE PITIGRDFEA LMNQHQDPLE VTQDVTREWA KKVVWKREKA SKINGAYCEG RVRGQAIRIR TMKMRQQASF LPATLTMTVD RGDNVNISFK KVLIKEEDAV IYKNGSIHSV PRHEVPDILE VHLPHAQPQD AGVYSARYIG GNLFTSAFTR LIVRRCEAQK WGPDCRPCTT CKNNGVCHED TGECICPPGF MGRTCEKACE PHTFGRTCKE RCSGPEGCKS YVFCPDPYGC SCATGWRGLQ CNEACPSGYY GPDCKLRCHC TNEEICDRFQ GCLCSQGWQG LQCKEGRPRM TPQIEDLPDH IEVNSGKFNP ICKASGWPLP TSEEMTLVKP DGTVLQPNDF NYDRFSVAIF TVNRVLPPDS GVWVCSVNTV AGMVEKPFNI SVKVLPEPLH APNVIDTGHN FIINISSEPY FGDGPIKSKK LFYKPVNQAW KYIEVTNEIF TLNYLEPRTD YELCVQLARP EGGEGHPGPV RRFTTASIGL PPPRGLSLLP KSQTALNLTW QPIFTNSEDE FYVEVERRSQ TTSDQQNIKV PGNLTSVLLS NLVPREQYTV RARVNTKAQG EWSEELRAWT LSDILPPQEN IKISNITDST AMVSWTIVDG YSISSIIIRY KVQGKNEDQH IDVKIKNATV TQYQLKGEPE TTYHVDIFAE NNIGSSNPAF SHELRTLPHS PASATRHHHH HH

Specificity: Chromosomal location:4 C5, 4 43.34 cM

Characteristics: Length (aa):712

Purity: > 95 % by SDS-PAGE

Application Details

Comment:

Soluble Receptors

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge vial prior to opening. The lyophilized sTIE-2-His is soluble in water and most aqueous buffers and should be reconstituted in PBS or medium to a concentration not lower than 50 μg/mL.

Buffer: PBS

Storage: -20 °C,-80 °C

Storage Comment: Lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted sTIE-2-His should be stored in working aliquots at -20°C.

Expiry Date: 6 months

Target Details

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Alternative Name: TIE-2 (TEK Products)

Synonyms: TEK Protein, DKFZp469E064 Protein, AA517024 Protein, Cd202b Protein, Hyk Protein, Tie2 Protein, tie-2 Protein, CD202B Protein, TIE-2 Protein, TIE2 Protein, VMCM Protein, VMCM1 Protein, Tie-2 Protein, tie2 Protein, TEK receptor tyrosine kinase Protein, angiopoietin-1 receptor Protein, TEK tyrosine kinase, endothelial Protein, TEK Protein, LOC100408334 Protein, tek Protein, Tek Protein

Background: Recombinant mouse soluble TIE-2 was fused with a 6x His-tag at the C-terminus. The soluble receptor protein consists of the full extracellular domain (Ala23-Ala737). Mouse sTIE-2 monomer has a calculated molecular mass of approximately 79,86 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 95 kDa protein in SDS-PAGE under reducing conditions. TIE-1 (tyrosine kinase with Ig and EGF homology domains 1) and TIE-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis.
Synonyms: Angiopoietin-1 receptor, Endothelial tyrosine kinase, HYK, STK1, Tunica interna endothelial cell kinase, Tyrosine kinase with Ig and EGF homology domains-2, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2, p140 TEK, CD202b, Tek, Hyk, Tie2, AA517024

Gene ID: 21687

NCBI Accession: NM_013690, NP_038718

UniProt: Q02858

Pathways: RTK Signaling, Growth Factor Binding