TEK Protein (glycosylated, Monomer, Soluble) (His tag)

Catalog No. ABIN1589752

Product Details

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: Monomer, glycosylated, Soluble

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This TEK protein is labelled with His tag.

Purpose: TIE-2, soluble

Sequence: AMDLILINSL PLVSDAETSL TCIASGWRPH EPITIGRDFE ALMNQHQDPL EVTQDVTREW AKKVVWKREK ASKINGAYFC EGRVRGEAIR IRTMKMRQQA SFLPATLTMT VDKGDNVNIS FKKVLIKEED AVIYKNGSFI HSVPRHEVPD ILEVHLPHAQ PQDAGVYSAR YIGGNLFTSA FTRLIVRRCE AQKWGPECNH LCTACMNNGV CHEDTGECIC PPGFMGRTCE KACELHTFGR TCKERCSGQE GCKSYVFCLP DPYGCSCATG WKGLQCNEAC HPGFYGPDCK LRCSCNNGEM CDRFQGCLCS PGWQGLQCER EGIPRMTPKI VDLPDHIEVN SGKFNPICKA SGWPLPTNEE MTLVKPDGTV LHPKDFNHTD HFSVAIFTIH RILPPDSGVW VCSVNTVAGM VEKPFNISVK VLPKPLNAPN VIDTGHNFAV INISSEPYFG DGPIKSKKLL YKPVNHYEAW QHIQVTNEIV TLNYLEPRTE YELCVQLVRR GEGGEGHPGP VRRFTTASIG LPPPRGLNLL PKSQTTLNLT WQPIFPSSED DFYVEVERRS VQKSDQQNIK VPGNLTSVLL NNLHPREQYV VRARVNTKAQ GEWSEDLTAW TLSDILPPQP ENIKISNITH SSAVISWTIL DGYSISSITI RYKVQGKNED QHVDVKIKNA TIIQYQLKGL EPETAYQVDI FAENNIGSSN PAFSHELVTL PESQAPADLG GGKTRHHHHH H

Specificity: Chromosomal location:9p21

Characteristics: Length (aa):731

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Measured by its ability to bind to immobilized recombinant Ang-2 in a functional ELISA.

Comment:

Soluble Receptors

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge vial prior to opening. The lyophilized sTIE-2-His is soluble in water and most aqueous buffers and should be reconstituted in PBS or medium to a concentration not lower than 50 μg/mL.

Buffer: PBS

Storage: -20 °C,-80 °C

Storage Comment: Lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted sTIE-2-His should be stored in working aliquots at -20°C.

Expiry Date: 6 months

Target Details

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Alternative Name: TIE-2 (TEK Products)

Synonyms: TEK Protein, DKFZp469E064 Protein, AA517024 Protein, Cd202b Protein, Hyk Protein, Tie2 Protein, tie-2 Protein, CD202B Protein, TIE-2 Protein, TIE2 Protein, VMCM Protein, VMCM1 Protein, Tie-2 Protein, tie2 Protein, TEK receptor tyrosine kinase Protein, angiopoietin-1 receptor Protein, TEK tyrosine kinase, endothelial Protein, TEK Protein, LOC100408334 Protein, tek Protein, Tek Protein

Background: Recombinant human soluble TIE-2/TEK was fused with a 6x His-tag at the C-terminus. The soluble receptor protein consists of the full extracellular domain (Thr19-Lys745). TIE-1 (tyrosine kinase with Ig and EGF homology domains 1) and TIE-2/TEK comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis. Human TIE-2 cDNA encodes a 1124 amino acid (aa) residue precursor protein with an 18 residue putative signal peptide, a 727 residue extracellular domain and a 354 residue cytoplasmic domain. Two ligands, angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2), which bind TIE-2 with high affinity have been identified. Ang2 has been reported to act as an antagonist for Ang1. Mice engineered to overexpress Ang2 or to lack Ang1 or TIE-2 display similar angiogenic defects.
Synonyms: EGFR, ERBB, HER1, mENA, ERBB1, PIG61

Molecular Weight: 95.0 kDa

Gene ID: 7010

NCBI Accession: NM_000459, NP_000450

UniProt: Q02763

Pathways: RTK Signaling, Growth Factor Binding