GREM1 Protein

Catalog No. ABIN1589732

Product Details

Target: GREM1 (Gremlin 1 (GREM1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purpose: Gremlin-1

Sequence: MKKKGSQGAI PPPDKAQHND SEQTQSPQQP GSRNRGRGQG RGTAMPGEEV LESSQEALHV TERKYLKRDW CKTQPLKQTI HEEGCNSRTI INRFCYGQCN SFYIPRHIRK EEGSFQSCSF CKPKKFTTMM VTLNCPELQP PTKKKRVTRV KQCRCISIDL D

Specificity: Chromosomal location:15q13.3

Characteristics: Length (aa):161

Purity: > 95 % by SDS-PAGE

Application Details

Comment:

Cytokines & Growth Factors

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Human Grem1 should be reconstituted in 50 mM acetic acid or sterile water to a concentration of 0.1 mg/mL. This solution can be diluted in water or other buffer solutions or stored at -20 °C.

Buffer: 50 mM acetic acid

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: RT,0 °C

Storage Comment: The lyophilized human Grem1, though stable at room temperature, is best stored desiccated below 0°C. Avoid repeated freeze-thaw cycles.

Target Details

Target: GREM1 (Gremlin 1 (GREM1))

Alternative Name: Gremlin-1 (GREM1 Products)

Synonyms: grem1 Protein, MGC136702 Protein, zgc:136702 Protein, GREM1 Protein, drm Protein, pig2 Protein, dand2 Protein, ihg-2 Protein, gremlin Protein, Cktsf1b1 Protein, Drm Protein, Grem Protein, ld Protein, gremlin-1 Protein, CKTSF1B1 Protein, DAND2 Protein, DRM Protein, GREMLIN Protein, IHG-2 Protein, cktsf1b1 Protein, gremlin 1a, DAN family BMP antagonist Protein, gremlin 1, DAN family BMP antagonist Protein, gremlin 1 Protein, gremlin 1, DAN family BMP antagonist L homeolog Protein, grem1a Protein, GREM1 Protein, LOC662541 Protein, grem1 Protein, Grem1 Protein, grem1.L Protein

Background: Gremlin, also known as “Increased in High Glucose protein 2” (IHG2) and “Down regulated in Mos-transformed cells protein” (Drm), is a 28 kDa member of the Dan family of secreted glycoproteins. Native human Gremlin consist of 160 amino acids. The mature region contains one potential site for N-linked glycosylation (Asn42), a cysteine-rich region, and a cysteine-knot motif (aa94-184) whose structure is shared by members of the TGFβ superfamily. Posttranslational modifications include glycosylation and phosphorylation (1-3). Human Gremlin exists in both secreted and membrane-associated forms and there exist 2 isoforms. The aa sequence identity of human Gremlin with mouse and chicken Gremlin is 99% and 86 %, respectively. Northern blot analysis shows that Gremlin mRNA is highly expressed in the small intestine, fetal brain and colon, and weakly expressed in adult brain, ovary, prostate, pancreas and skeletal muscle. Gremlin functions as a bone morphogenetic protein (BMP) antagonist. It acts by binding to, and forming heterodimers with, BMP2, BMP4, and BMP7, thus preventing them from interacting with their cell surface receptors. This mechanism is thought to be responsible for the pattern-inducing activity of Gremlin during embryonic development and to play a role in human diseases, such as diabetic nephropathy). However, intracellular BMP-independent mechanisms of action may mediate the ability of Gremlin to suppress transformation and tumor genesis under certain experimental conditions. Gremlin also interacts with Slit proteins and acts as an inhibitor of monocyte chemotaxis. In addition, Gremlin has been found to be a proangiogenic factor expressed by endothelium. Furthermore Gremlin is a novel agonist of the major proangiogenic receptor VEGFR2.
Synonyms: GREM1, DRM, PIG2, DAND2, IHG-2, GREMLIN, CKTSF1B1, Cell proliferation-inducing gene 2 protein, Cysteine knot superfamily 1, BMP antagonist 1, DAN domain family member 2, Down-regulated in Mos-transformed cells protein, Increased in high glucose protein 2

Molecular Weight: 18.4 kDa

Gene ID: 26585

NCBI Accession: NM_013372, NP_037504

UniProt: O60565

Pathways: Regulation of Muscle Cell Differentiation, Tube Formation, Maintenance of Protein Location