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GM-CSF Protein

CSF2 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 98 % by SDS-PAGE Active
Catalog No. ABIN1589646
  • Target See all GM-CSF (CSF2) Proteins
    GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
    Protein Type
    Recombinant
    Biological Activity
    Active
    Origin
    • 23
    • 11
    • 8
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Source
    • 23
    • 9
    • 8
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    Escherichia coli (E. coli)
    Purpose
    GM-CSF
    Sequence
    APARSPSPST QPWEHVNAIQ EARRLLNLSR DTAAEMNETV EVISEMFDLQ EPTCLQTRLE LYKQGLRGSL TKLKGPLTMM ASHYKQHCPP TPETSCATQI ITFESFKENL KDFLLVIPFD CWEPVQE
    Specificity
    Chromosomal location:5q31.1
    Characteristics
    Length (aa):127
    Purity
    > 98 % by SDS-PAGE
    Endotoxin Level
    < 0.1 ng per μg of GM-CSF
    Top Product
    Discover our top product CSF2 Protein
  • Application Notes
    The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is <0.1 ng/mL corresponding to a specific activity of ≥1 x 10^7 units/mg.
    Comment

    Cytokines & Growth Factors

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    The lyophilized rh GM-CSF is soluble in water and most aqueous buffers and can be reconstituted in water to a concentration of 0.1 mg/mL. This solution can be diluted into other buffered solutions or stored at -20 °C for future use.
    Buffer
    PBS, pH 7.2
    Storage
    RT,-20 °C
    Storage Comment
    The lyophilized powder although stable at room temperature for 3 weeks, is best stored desiccated at -20°C. Reconstituted GM-CSF should be stored in working aliquots at -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
  • Target
    GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
    Alternative Name
    GM-CSF (CSF2 Products)
    Synonyms
    GMCSF Protein, GM-CSF Protein, CSF2 Protein, Csfgm Protein, Gm-CSf Protein, MGI-IGM Protein, Gm-csf Protein, Gmcsf Protein, CSF Protein, colony stimulating factor 2 Protein, colony stimulating factor 2 (granulocyte-macrophage) Protein, CSF2 Protein, Csf2 Protein
    Background
    Recombinant human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), a 14,5 kDa protein consisting of 127 amino acid residues (Ala18-Glu144), is a potent species specific stimulator of bone marrow cells and several other cell types. GM-CSF was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages and eosinophils. GM-CSF has also been reported to have a functional role on non-hematopoietic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines. GM-CSF is species specific and human GM-CSF has no biological effects on mouse cells. GM-CSF exerts its biological effects through binding to specific cell surface receptors. The high affinity receptors required for human GM-CSF signal transduction have been shown to be heterodimers consisting of a GM-CSF-specific α chain and a common β chain that is shared by the high-affinity receptors for IL-3 and IL-5.
    Synonyms: CSF2, GM-CSF
    Molecular Weight
    14.5 kDa
    Gene ID
    1437
    NCBI Accession
    NM_000758, NP_000749
    Pathways
    JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin
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