IL-6 Protein

Catalog No. ABIN1589641

Product Details

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purpose: IL-6

Sequence: MAPVPPGEDS KDVAAPHRQP LTSSERIDKQ IRYILDGISA LRKETCNKSN MCESSKEALA ENNLNLPKMA EKDGCFQSGF NEETCLVKII TGLLEFEVYL EYLQNRFESS EEQARAVQMS TKVLIQFLQK KAKNLDAITT PDPTTNASLL TKLQAQNQWL QDMTTHLILR SFKEFLQSSL RALRQM

Specificity: Chromosomal location:7p21

Characteristics: Length (aa):186

Purity: > 98 % by SDS-PAGE

Endotoxin Level: < 0.1 ng per μg (IEU/μg) of rh IL-6

Application Details

Application Notes: The ED50 as determined by the dose-dependent stimulation of murine hybridoma B9 cells is in the range of ≤ 10 - 25 pg/mL.

Comment:

Cytokines & Growth Factors

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: The lyophilized IL-6 should be reconstituted in water to a concentration not less than 100 μg/mL. This solution can be diluted into other buffered solutions or stored at -20 °C for future use.

Buffer: PBS

Storage: RT,0 °C,-20 °C

Storage Comment: The lyophilized IL-6, though stable at room temperature, is best stored desiccated below 0°C. Reconstituted IL-6 should be stored in working aliquots at -20°C.

References

Poli, Asperti, Ruzzenenti, Mandelli, Campostrini, Martini, Di Somma, Maccarinelli, Girelli, Naggi, Arosio: "Oversulfated heparins with low anticoagulant activity are strong and fast inhibitors of hepcidin expression in vitro and in vivo." in: Biochemical pharmacology, Vol. 92, Issue 3, pp. 467-75, (2014) (PubMed).

Target Details

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Alternative Name: IL-6 (IL6 Products)

Synonyms: BSF2 Protein, HGF Protein, HSF Protein, IFNB2 Protein, IL-6 Protein, Il-6 Protein, ILg6 Protein, Ifnb2 Protein, il6 Protein, CHIL-6 Protein, interleukin 6 Protein, interleukin-6 Protein, IL6 Protein, Il6 Protein, il-6 Protein, IL-6 Protein

Background: Interleukin 6 (IL-6) is a pleiotropic α-helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is essential for the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22 kDa-28 kDa phosphorylated and variably glycosylated molecule. Mature human IL6 is 183 amino acids (aa) in length and shares 41 % aa sequence identity with mouse and rat IL-6. Alternate splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties. Human IL6 is equally active on mouse and rat cells. IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization. Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130expressing cells that lack cell surface IL-6 R. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/ IL-6 R but not from other cytokines that utilize gp130 as a co-receptor.
Synonyms: IL6, HGF, HSF, BSF2, IL-6, IFNB2

Molecular Weight: 21.1 kDa

Gene ID: 3569

NCBI Accession: NM_000600, NP_000591

UniProt: P05231

Pathways: TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome