PDGFC Protein (Homodimer)

Catalog No. ABIN1589585

Product Details

Target: PDGFC (Platelet-Derived Growth Factor C (PDGFC))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: Homodimer

Origin: Parapoxvirus

Source: Escherichia coli (E. coli)

Purpose: VEGF-E

Sequence: MGSSHHHHHH SSGLVPRGSH DSTKTWSEVF ENSGCKPRPM VFRVHDEHPE LTSQRFNPPC VTLMRCGGCC NDESLECVPT EEANVTMQLM GASVSGGNGM QHLSFVEHKK CDCKPPLTTT PPTTTRPPRR RR

Characteristics: Length (aa):132

Purity: > 90 % by SDS-PAGE

Endotoxin Level: < 0.1 ng per μg of ov-VEGF-E

Application Details

Application Notes: Measured in a cell proliferation assay using primary HUVECs. The ED50 for this effect is typically 1 - 5 ng/mL.

Comment:

Cytokines & Growth Factors

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: The lyophilized ovVEGF-E should be reconstituted in water or medium to a concentration not lower than 50 μg/mL. For long term storage we would recommend to add at least 0.1 % human or bovine serum albumin.

Buffer: PBS

Target Details

Target: PDGFC (Platelet-Derived Growth Factor C (PDGFC))

Alternative Name: VEGF-E (PDGFC Products)

Synonyms: SCDGF Protein, FALLOTEIN Protein, 1110064L01Rik Protein, AI647969 Protein, PDGF-C Protein, platelet derived growth factor C Protein, platelet-derived growth factor, C polypeptide Protein, PDGFC Protein, Pdgfc Protein

Target Type: Viral Protein

Background: A DNA sequence encoding the mature variant of ovVEGF-E isolate D1701 (Dehio et al., 1999, GenBank accession No. AF106020) was expressed in E. coli as a 132 amino acid residue fusion protein with an N-terminal His-tag sequence and a thrombin cleavage site. Recombinant VEGF-E homodimer was dimerized in vitro and has a predicted mass of approximately 35 kDa. Based on sequence similarity to VEGF-A, a gene encoding a VEGF homologue has recently been discovered in the genome of Orf virus (OV) (Lyttle et al., 1994). Different isolates of Orf virus show significant amino acid sequence similarity to VEGF-A and described as a viral virulence factor that appears to be derived from captured host genes. All eight cysteine residues of the central cysteine knot motif characteristic of members of the VEGF family are conserved among other residues in the VEGF-E proteins (Dehio et al., 1999, Wise et al., 1999). Alignment of all mammalian VEGF sequences indicated that VEGF-E is distinct from the previously described VEGFs but most closely related to VEGF-A. Like VEGF-A, VEGF-E was found to bind with high affinity to VEGF receptor-2 (KDR) resulting in receptor autophosphorylation, whilst in contrast to VEGF-A, VEGF-E can not bind to VEGF receptor-1 (Flt-1). Furthermore VEGF-E can also not bind to VEGF receptor-3 (FLT-4). Therefore VEGF-E is a potent angiogenic factor selectively binding to VEGF receptor –2/KDR.
Synonyms: Vascular Endothelial Growth Factor-E

Molecular Weight: 35 kDa (Dimer)

UniProt: Q9YMF3

Pathways: RTK Signaling, Platelet-derived growth Factor Receptor Signaling