FLT4 Protein (Dimer, glycosylated, Soluble) (Fc Tag)

Catalog No. ABIN1589551

Product Details

Target: FLT4 (Fms-Related Tyrosine Kinase 4 (FLT4))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: glycosylated, Dimer, Soluble

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This FLT4 protein is labelled with Fc Tag.

Purpose: VEGFR-3/FLT-4/Fc Chimera, soluble

Sequence: YSMTPPTLNI TEDSYVIDTG DSLSISCRGQ HPLEWTWPGA QEVLTTGGKD SEDTRVVHDC EGTEARPYCK VLLLAQTHAN NTGSYHCYYK YIKARIEGTT AASTYVFVRD FKHPFINKPD TLLVNRKDSM WVPCLVSIPG LNITLRSQSS ALHPDGQEVL WDDRRGMRVP TQLLRDALYL QCETTWGDQN FLSNLFVVHI TGNELYDIQL YPKKSMELLV GEKLVLNCTV WAEFDSGVTF DWDYPGKQAE RAKWVPERRS QQTHTELSSI LTIHNVSQND LGPYVCEANN GIQRFRESTE VIVHEKPFIS VEWLKGPVLE ATAGDELVKL PVKLAAYPPP EFQWYKDRKA VTGRHNPHAL VLKEVTEASA GVYTLALWNS AAGLRQNISL ELVVNVPPHI HEKEASSPSI YSRHSRQTLT CTAYGVPQPL SVQWHWRPWT PCKTFAQRSL RRRQQRDGMP QCRDWKEVTT QDAVNPIESL DSWTEFVEGK NKTVSKLVIQ DANVSAMYKC VVVNKVGQDE RLIYFYVTTI PDGFSIESEP SEDPLEGQSV RLSCRADNYT YEHLRWYRLN LSTLHDAQGN PLLLDCKNVH LFATPLEANL EEAEPGARHA TLSLNIPRVA PEDEGDYVCE VQDRRSQDKH CHKKYLSVQA LEAPRLTQNL TDLLVNVSDS LEMRCPVAGA HVPSIVWYKD ERLLEKESGI DLADSNQRLS IQRVREEDAG RYLCSVCNAK GCVNSSASVA VEGSEDKGSM ESDKTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV YTLPPSREEM TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPML DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGK

Specificity: Chromosomal location:5q35.3

Characteristics: Length (aa):979

Purity: > 90 % by SDS-PAGE

Application Details

Application Notes: Measured by its ability to bind recombinant rat VEGF-C in a functional solid phase binding assay. Immobilised recombinant human sVEGFR-3/Fc at 5 μg/mL can bind recombinant rat VEGF-C in a linear range of 8-500 ng/mL.

Comment:

Soluble Receptors

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: The lyophilized sVEGFR-3/Fc is soluble in water and most aqueous buffers and should be reconstituted in PBS or medium to a concentration not lower than 100 μg/mL.

Buffer: PBS

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C,-80 °C

Storage Comment: Lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted sVEGFR-3/Fc should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles!

Expiry Date: 6 months

Target Details

Target: FLT4 (Fms-Related Tyrosine Kinase 4 (FLT4))

Alternative Name: VEGFR-3/FLT-4 (FLT4 Products)

Synonyms: FLT41 Protein, LMPH1A Protein, PCL Protein, VEGFR3 Protein, AI323512 Protein, Chy Protein, Flt-4 Protein, VEGFR-3 Protein, Vegfr3 Protein, cb1059 Protein, fms related tyrosine kinase 4 Protein, FMS-like tyrosine kinase 4 Protein, fms-related tyrosine kinase 4 Protein, FLT4 Protein, Flt4 Protein, flt4 Protein

Background: Recombinant human soluble Vascular Endothelial Growth Factor Receptor-3 (sVEGFR-3) was fused with the Fc part of human IgG1. The recombinant mature sVEGFR-3/Fc is a disulfide-linked homodimeric protein. The sVEGFR-3/Fc monomers have a mass of approximately 130 kDa. The soluble receptor protein consists of all 7 extracellular domains (Met1-Glu774). All three VEGF receptors belong to the class III subfamily of receptor tyrosine kinases (RTKs) characterised by the seven immunoglobulin-like loops in the extracellular domain. The expression of VEGFR-1 to -3 is almost exclusively restricted to hematopoietic precursor cells, vascular and lymphatic endothelial cells and to the monocyte/macrophage lineage. They play key roles in vasculogenesis, hematopoiesis, angiogenesis and lymphangiogenesis. The VEGFR-3/FLT-4 cDNA encodes a 1298 amino acid (aa) residue precursor protein with a 23aa residue signal peptide. Mature VEGFR-3/FLT-4 is composed of a 751aa residue extracellular domain, a 22aa transmembrane domain and a 482aa residue cytoplasmic domain. Both VEGF family members VEGF-C and VEGF-D have been shown to bind and activate VEGFR-3/FLT-4. The FLT-4 gene is widely expressed in the early embryo but becomes restricted to the lymphatic endothelial at latter stages of development. It is important for lymphangiogenesis.
Synonyms: soluble vascular endothelial growth factor receptor-3, FLT4, PCL, LMPH1A, fms-related tyrosine kinase 4

Molecular Weight: ~ 130.0 kDa

Gene ID: 2324

NCBI Accession: NM_002020, NP_002011

UniProt: P35916

Pathways: RTK Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGF Signaling