Protein A Protein (Biotin)

Catalog No. ABIN1536532

Product Details

Target: Protein A

Origin: Staphylococcus aureus

Host: Please inquire

Purification tag / Conjugate: This Protein A protein is labelled with Biotin.

Application: ELISA, Western Blotting (WB), Dot Blot (DB), Immunohistochemistry (IHC)

Application Details

Application Notes: Working concentrations for specific applications should be determined by titration assay. The chart below may serve as a useful guideline, but primary antibody, temperature, incubation time, the amount of antigen, and the sensitivity of the detection methods can all affect the ideal working concentration. Dot blot: 1:5,000-1:50,000 Western blot: 1:5,000-1:50,000 ELISA: 1:50,000-1:500,000 Other: user-optimized.

Restrictions: For Research Use only

Handling

Concentration: 2 mg/ml

Buffer: PBS (pH 7.4) containing 10 mg/ml BSA and 0.02% sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4°C for short periods of time, one month or shorter. Aliquot and store at -20°C for longer periods. Avoid repeated freeze/thaw cycles. Prolonged storage may result in slight precipitation, so clarify the solution by centrifugation before use.

References

Wen, He, Lee: "Specific antibody immobilization with biotin-poly(L-lysine)-g-poly(ethylene glycol) and protein A on microfluidic chips." in: Journal of immunological methods, Vol. 350, Issue 1-2, pp. 97-105, (2009) (PubMed).

Target Details

Target: Protein A

Abstract: Protein A Products

Synonyms: alpha protein Protein, repB Protein

Background: Staphylococcal Protein A binds strongly and specifically to the Fc region of immunoglobulin molecules, particularly IgG. The product contains five high-affinity binding sites capable of interacting with the Fc region of IgG in humans, rabbits, pigs, and other species. Biotin-Protein A, which is produced by covalently linking biotin to protein A, can be used in immunodetections, such as immunoblotting (western or dot blot), ELISA, and immunohistochemistry.