Protein A Protein (HRP)

Catalog No. ABIN1536531

Product Details

Target: Protein A

Origin: Staphylococcus aureus

Host: Please inquire

Purification tag / Conjugate: This Protein A protein is labelled with HRP.

Application: ELISA, Western Blotting (WB), Dot Blot (DB), Immunohistochemistry (IHC)

Application Details

Application Notes: This product is suitable for immunoblotting (Western or Dot blot), ELISA, immunoperoxidase electron microscopy, and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. The investigator must determine the ideal working concentration for each specific application. We have not yet determined the suitability of this antibody for applications other than those listed below. The ideal working concentration must take into account such factors as secondary antibody affinity, antigen concentration, sensitivity of the detection method, temperature, and the length of the incubations. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. Dot blot: 1:5,000-1:20,000Western blot: 1:5,000-1:20,000ELISA: 1:50,000-1:250,000Immunohistology: 1:500-1:2,500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized power with 1.0 ml of deionized water (or equivalent) to a final HRP-Protein A concentration of 1 mg/ml.

Buffer: PBS, pH 7.4, containing 1% BSA and 0.01% Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: HRP-Protein A should be stored lyophilized until use. It remains stable in lyophilized form for at least one year if stored at 2-8°C. The reconstituted product can be stored for 2-3 weeks at 2-8°C or for up to one year at -20°C or below. Avoid repeated freezing and thawing cycles.

Expiry Date: 12 months

References

Rebois, Maki, Meeks, Fishman, Hébert, Northup: "D2-like dopamine and ?-adrenergic receptors form a signaling complex that integrates Gs- and Gi-mediated regulation of adenylyl cyclase." in: Cellular signalling, Vol. 24, Issue 11, pp. 2051-60, (2012) (PubMed).

Bohgaki, Mozo, Salmena, Matysiak-Zablocki, Bohgaki, Sanchez, Strasser, Hakem, Hakem: "Caspase-8 inactivation in T cells increases necroptosis and suppresses autoimmunity in Bim-/- mice." in: The Journal of cell biology, Vol. 195, Issue 2, pp. 277-91, (2011) (PubMed).

Target Details

Target: Protein A

Abstract: Protein A Products

Background: Staphylococcal Protein A binds strongly and specifically to the Fc region of immunoglobulin molecules, especially IgG of many species such as Human, Rabbit, Pig, etc. The product contains five high affinity binding sites that are capable of interacting with the Fc region of IgG. HRP-Protein A is produced by covalently linking HRP to Protein A, which can be used in immuno-detection such as immunoblotting (Western or Dot blot), ELISA, and immunohistochemistry.